Repository logo
 

Cerebral organoids with chromosome 21 trisomy secrete Alzheimer's disease-related soluble aggregates detectable by single-molecule-fluorescence and super-resolution microscopy.

Published version
Peer-reviewed

Repository DOI


Change log

Authors

Fertan, Emre 
Böken, Dorothea 
Danial, John SH 
Lam, Jeff YL 

Abstract

Understanding the role of small, soluble aggregates of beta-amyloid (Aβ) and tau in Alzheimer's disease (AD) is of great importance for the rational design of preventative therapies. Here we report a set of methods for the detection, quantification, and characterisation of soluble aggregates in conditioned media of cerebral organoids derived from human iPSCs with trisomy 21, thus containing an extra copy of the amyloid precursor protein (APP) gene. We detected soluble beta-amyloid (Aβ) and tau aggregates secreted by cerebral organoids from both control and the isogenic trisomy 21 (T21) genotype. We developed a novel method to normalise measurements to the number of live neurons within organoid-conditioned media based on glucose consumption. Thus normalised, T21 organoids produced 2.5-fold more Aβ aggregates with a higher proportion of larger (300-2000 nm2) and more fibrillary-shaped aggregates than controls, along with 1.3-fold more soluble phosphorylated tau (pTau) aggregates, increased inflammasome ASC-specks, and a higher level of oxidative stress inducing thioredoxin-interacting protein (TXNIP). Importantly, all this was detectable prior to the appearance of histological amyloid plaques or intraneuronal tau-pathology in organoid slices, demonstrating the feasibility to model the initial pathogenic mechanisms for AD in-vitro using cells from live genetically pre-disposed donors before the onset of clinical disease. Then, using different iPSC clones generated from the same donor at different times in two independent experiments, we tested the reproducibility of findings in organoids. While there were differences in rates of disease progression between the experiments, the disease mechanisms were conserved. Overall, our results show that it is possible to non-invasively follow the development of pathology in organoid models of AD over time, by monitoring changes in the aggregates and proteins in the conditioned media, and open possibilities to study the time-course of the key pathogenic processes taking place.

Description

Acknowledgements: DK is funded by UK Dementia Research Institute, which receives its funding from DRI Ltd. funded by the UK Medical Research Council and the Royal Society. DN is funded by The Wellcome Trust Collaborative Award in Science 217199/Z/19/Z. AM was awarded a William Harvey Academy Fellowship, co-funded by the People Programme (Marie Curie Actions) under REA no. 608765 and also a Jérôme Lejeune Foundation Post-doctoral Fellowship. IA was awarded an Adris Foundation grant. JYLL is supported by the Croucher Foundation Limited (Hong Kong).


Funder: Royal Society; doi: https://doi.org/10.13039/501100000288


Funder: D.K. is funded by UK Dementia Research Institute, which receives its funding from DRI Ltd. funded by the UK Medical Research Council.


Funder: A.M. was awarded a William Harvey Academy Fellowship, co-funded by the People Programme (Marie Curie Actions) under REA no. 608765 and also a Jérôme Lejeune Foundation Post-doctoral Fellowship.


Funder: J.Y.L.L. is supported by the Croucher Foundation Limited (Hong Kong).


Funder: I.A. was awarded an Adris Foundation grant.

Keywords

Humans, Organoids, Alzheimer Disease, tau Proteins, Down Syndrome, Induced Pluripotent Stem Cells, Amyloid beta-Peptides, Amyloid beta-Protein Precursor, Neurons, Brain, Carrier Proteins, Trisomy, Oxidative Stress, Plaque, Amyloid, Culture Media, Conditioned, Microscopy, Fluorescence

Journal Title

Mol Psychiatry

Conference Name

Journal ISSN

1359-4184
1476-5578

Volume Title

29

Publisher

Springer Science and Business Media LLC
Sponsorship
European Commission (608765)
D.K. is funded by UK Dementia Research Institute, which receives its funding from DRI Ltd. funded by the UK Medical Research Council and the Royal Society. D.N. is funded by The Wellcome Trust Collaborative Award in Science 217199/Z/19/Z. A.M. was awarded a William Harvey Academy Fellowship, co-funded by the People Programme (Marie Curie Actions) under REA no. 608765 and also a Jérôme Lejeune Foundation Post-doctoral Fellowship. I.A. was awarded an Adris Foundation grant. J.Y.L.L. is supported by the Croucher Foundation Limited (Hong Kong).