Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency.


Type
Article
Change log
Authors
Neganova, Irina 
Cotts, Lewis 
Banks, Peter 
Gassner, Katja 
Shukurov, Anvar 
Abstract

Direct reprogramming of human somatic cells toward induced pluripotent stem cells holds great promise for regenerative medicine and basic biology. We used a high-throughput small interfering RNA screening assay in the initiation phase of reprogramming for 784 genes belonging to kinase and phosphatase families and identified 68 repressors and 22 effectors. Six new candidates belonging to the family of the G protein-coupled receptors (GPCRs) were identified, suggesting an important role for this key signaling pathway during somatic cell-induced reprogramming. Downregulation of one of the key GPCR effectors, endothelial differentiation GPCR5 (EDG5), impacted the maintenance of pluripotency, actin cytoskeleton organization, colony integrity, and focal adhesions in human embryonic stem cells, which were associated with the alteration in the RhoA-ROCK-Cofilin-PAXILLIN-actin signaling pathway. Similarly, downregulation of EDG5 during the initiation stage of somatic cell-induced reprogramming resulted in alteration of cytoskeleton, loss of human-induced pluripotent stem cell colony integrity, and a significant reduction in partially and fully reprogrammed cells as well as the number of alkaline phosphatase positive colonies at the end of the reprogramming process. Together, these data point to an important role of EDG5 in the maintenance and acquisition of pluripotency. Stem Cells 2019;37:318-331.

Description
Keywords
Cytoskeleton, EDG5, GPCR, Genome-wide RNAi screen, Human somatic cell reprogramming, hESCs/hiPSCs, Cell Line, Cellular Reprogramming, Down-Regulation, Human Embryonic Stem Cells, Humans, Induced Pluripotent Stem Cells, Signal Transduction, Sphingosine-1-Phosphate Receptors
Journal Title
Stem Cells
Conference Name
Journal ISSN
1066-5099
1549-4918
Volume Title
37
Publisher
Oxford University Press (OUP)
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/M00015X/2)