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The subcellular organisation of Saccharomyces cerevisiae.

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cam.issuedOnline2018-11-29
cam.orpheus.successThu Jan 30 10:52:50 GMT 2020 - The item has an open VoR version.
dc.contributor.authorNightingale, Daniel Jh
dc.contributor.authorGeladaki, Aikaterini
dc.contributor.authorBreckels, Lisa M
dc.contributor.authorOliver, Stephen G
dc.contributor.authorLilley, Kathryn S
dc.contributor.orcidNightingale, Daniel [0000-0002-8081-8755]
dc.contributor.orcidOliver, Stephen [0000-0001-6330-7526]
dc.contributor.orcidLilley, Kathryn [0000-0003-0594-6543]
dc.date.accessioned2019-01-04T08:44:01Z
dc.date.available2019-01-04T08:44:01Z
dc.date.issued2019-02
dc.description.abstractSubcellular protein localisation is essential for the mechanisms that govern cellular homeostasis. The ability to understand processes leading to this phenomenon will therefore enhance our understanding of cellular function. Here we review recent developments in this field with regard to mass spectrometry, fluorescence microscopy and computational prediction methods. We highlight relative strengths and limitations of current methodologies focussing particularly on studies in the yeast Saccharomyces cerevisiae. We further present the first cell-wide spatial proteome map of S. cerevisiae, generated using hyperLOPIT, a mass spectrometry-based protein correlation profiling technique. We compare protein subcellular localisation assignments from this map, with two published fluorescence microscopy studies and show that confidence in localisation assignment is attained using multiple orthogonal methods that provide complementary data.
dc.description.sponsorshipBBSRC and Wellcome Trust
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.34831
dc.identifier.eissn1879-0402
dc.identifier.issn1367-5931
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287525
dc.languageeng
dc.language.isoeng
dc.publisherElsevier BV
dc.publisher.urlhttp://dx.doi.org/10.1016/j.cbpa.2018.10.026
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMass Spectrometry
dc.subjectMicroscopy, Fluorescence
dc.subjectProteomics
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.titleThe subcellular organisation of Saccharomyces cerevisiae.
dc.typeArticle
dcterms.dateAccepted2018-10-31
prism.endingPage95
prism.publicationDate2019
prism.publicationNameCurr Opin Chem Biol
prism.startingPage86
prism.volume48
pubs.funder-project-idWellcome Trust (108441/Z/15/Z)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/N023129/1)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/K00137X/1)
pubs.funder-project-idWellcome Trust (110170/Z/15/Z)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L002817/1)
rioxxterms.licenseref.startdate2019-02
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1016/j.cbpa.2018.10.026

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