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Glycogen synthase kinase 3 (GSK-3) controls T-cell motility and interactions with antigen presenting cells.

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Taylor, Alison 
Rudd, Christopher E  ORCID logo


OBJECTIVE: The threonine/serine kinase glycogen synthase kinase 3 (GSK-3) targets multiple substrates in T-cells, regulating the expression of Tbet and PD-1 on T-cells. However, it has been unclear whether GSK-3 can affect the motility of T-cells and their interactions with antigen presenting cells. RESULTS: Here, we show that GSK-3 controls T-cell motility and interactions with other cells. Inhibition of GSK-3, using structurally distinct inhibitors, reduced T-cell motility in terms of distance and displacement. While SB415286 reduced the number of cell-cell contacts, the dwell times of cells that established contacts with other cells did not differ for T-cells treated with SB415286. Further, the increase in cytolytic T-cell (CTL) function in killing tumor targets was not affected by the inhibition of motility. This data shows that the inhibition of GSK-3 has differential effects on T-cell motility and CTL function where the negative effects on cell-cell interactions is overridden by the increased cytolytic potential of CTLs.



Cell contacts, GSK-3, Motility, T-cells, Aminophenols, Animals, Antigen-Presenting Cells, Cell Movement, Glycogen Synthase Kinase 3, Maleimides, Mice, Mice, Inbred C57BL, T-Lymphocytes, Cytotoxic

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Springer Science and Business Media LLC
Wellcome Trust (092627/Z/10/Z)