Translocation frequency of double-stranded DNA through a solid-state nanopore.
Change log
Authors
Bell, Nicholas AW
Muthukumar, Murugappan
Keyser, Ulrich F
Abstract
Solid-state nanopores are single-molecule sensors that measure changes in ionic current as charged polymers such as DNA pass through. Here, we present comprehensive experiments on the length, voltage, and salt dependence of the frequency of double-stranded DNA translocations through conical quartz nanopores with mean opening diameter 15 nm. We observe an entropic barrier-limited, length-dependent translocation frequency at 4M LiCl salt concentration and a drift-dominated, length-independent translocation frequency at 1M KCl salt concentration. These observations are described by a unifying convection-diffusion equation, which includes the contribution of an entropic barrier for polymer entry.
Description
Keywords
Base Pairing, DNA, Movement, Nanopores, Potassium Chloride
Journal Title
Phys Rev E
Conference Name
Journal ISSN
2470-0045
2470-0053
2470-0053
Volume Title
93
Publisher
American Physical Society (APS)
Publisher DOI
Sponsorship
European Research Council (261101)