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Distinct routes of lineage development reshape the human blood hierarchy across ontogeny.



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Notta, Faiyaz 
Zandi, Sasan 
Takayama, Naoya 
Dobson, Stephanie 
Gan, Olga I 


In a classical view of hematopoiesis, the various blood cell lineages arise via a hierarchical scheme starting with multipotent stem cells that become increasingly restricted in their differentiation potential through oligopotent and then unipotent progenitors. We developed a cell-sorting scheme to resolve myeloid (My), erythroid (Er), and megakaryocytic (Mk) fates from single CD34(+) cells and then mapped the progenitor hierarchy across human development. Fetal liver contained large numbers of distinct oligopotent progenitors with intermingled My, Er, and Mk fates. However, few oligopotent progenitor intermediates were present in the adult bone marrow. Instead, only two progenitor classes predominate, multipotent and unipotent, with Er-Mk lineages emerging from multipotent cells. The developmental shift to an adult "two-tier" hierarchy challenges current dogma and provides a revised framework to understand normal and disease states of human hematopoiesis.



Adult, Antigens, CD34, Cell Lineage, Cell Separation, Cells, Cultured, Erythroid Cells, Fetal Blood, Gene Expression Profiling, Hematopoiesis, Humans, Liver, Megakaryocyte Progenitor Cells, Megakaryocytes, Multipotent Stem Cells, Myeloid Cells, Transcription, Genetic

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American Association for the Advancement of Science (AAAS)
Wellcome Trust (107630/Z/15/Z)
Wellcome Trust (097922/Z/11/B)
This work was supported by Postdoctoral Fellowship Awards from Canadian Institute of Health Research (CIHR) to FN and SZ. SZ is supported by (Aplastic Anemia). FN is a recipient of a scholar’s research award from the Ontario Institute of Cancer Research (OICR), through generous support from the Ontario Ministry of Research and Innovation. Research in EL laboratory is supported by a Wellcome Trust Sir Henry Dale Fellowship and core support grant from the Wellcome Trust and MRC to the Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute. Work in the Dick laboratory is supported by grants from the CIHR, Canadian Cancer Society, Terry Fox Foundation, Genome Canada through the Ontario Genomics Institute, OICR with funds from the province of Ontario, a Canada Research Chair and the Ontario Ministry of Health and Long Term Care (OMOHLTC).