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Balanced gene dosage control rather than parental origin underpins genomic imprinting.

Published version
Peer-reviewed

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Authors

Weinberg-Shukron, Ariella 
Ben-Yair, Raz 
Takahashi, Nozomi 
Dunjić, Marko 

Abstract

Mammalian parental imprinting represents an exquisite form of epigenetic control regulating the parent-specific monoallelic expression of genes in clusters. While imprinting perturbations are widely associated with developmental abnormalities, the intricate regional interplay between imprinted genes makes interpreting the contribution of gene dosage effects to phenotypes a challenging task. Using mouse models with distinct deletions in an intergenic region controlling imprinting across the Dlk1-Dio3 domain, we link changes in genetic and epigenetic states to allelic-expression and phenotypic outcome in vivo. This determined how hierarchical interactions between regulatory elements orchestrate robust parent-specific expression, with implications for non-imprinted gene regulation. Strikingly, flipping imprinting on the parental chromosomes by crossing genotypes of complete and partial intergenic element deletions rescues the lethality of each deletion on its own. Our work indicates that parental origin of an epigenetic state is irrelevant as long as appropriate balanced gene expression is established and maintained at imprinted loci.

Description

Funder: Minerva Foundation (Minerva Stiftung); doi: https://doi.org/10.13039/501100001658


Funder: Israel Cancer Research Fund (Israel Cancer Research Fund, Inc.); doi: https://doi.org/10.13039/100001698


Funder: Moross Integrated Cancer Center, Schwartz/Reisman Collaborative Science Program

Keywords

Article, /631/208/176/1968, /631/136/2442, /631/208/200, /631/208/176/1433, /631/208/176/1988, /38/22, /38/23, /38/39, /38/77, /38/88, /38/90, /42/100, /64/60, /82/51, /96/63, /13/106, /13/109, /59/5, article

Journal Title

Nat Commun

Conference Name

Journal ISSN

2041-1723
2041-1723

Volume Title

Publisher

Springer Science and Business Media LLC
Sponsorship
Medical Research Council (MR/R009791/1)
Wellcome Trust (210757/Z/18/Z)