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Integration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots.

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cam.issuedOnline2013-12-31
dc.contributor.authorMuraro, Daniele
dc.contributor.authorMellor, Nathan
dc.contributor.authorPound, Michael P
dc.contributor.authorHelp, Hanna
dc.contributor.authorLucas, Mikaël
dc.contributor.authorChopard, Jérôme
dc.contributor.authorByrne, Helen M
dc.contributor.authorGodin, Christophe
dc.contributor.authorHodgman, T Charlie
dc.contributor.authorKing, John R
dc.contributor.authorPridmore, Tony P
dc.contributor.authorHelariutta, Ykä
dc.contributor.authorBennett, Malcolm J
dc.contributor.authorBishopp, Anthony
dc.contributor.orcidHelariutta, Yrjo [0000-0002-7287-8459]
dc.date.accessioned2018-10-18T10:21:55Z
dc.date.available2018-10-18T10:21:55Z
dc.date.issued2014-01-14
dc.description.abstractAs multicellular organisms grow, positional information is continually needed to regulate the pattern in which cells are arranged. In the Arabidopsis root, most cell types are organized in a radially symmetric pattern; however, a symmetry-breaking event generates bisymmetric auxin and cytokinin signaling domains in the stele. Bidirectional cross-talk between the stele and the surrounding tissues involving a mobile transcription factor, SHORT ROOT (SHR), and mobile microRNA species also determines vascular pattern, but it is currently unclear how these signals integrate. We use a multicellular model to determine a minimal set of components necessary for maintaining a stable vascular pattern. Simulations perturbing the signaling network show that, in addition to the mutually inhibitory interaction between auxin and cytokinin, signaling through SHR, microRNA165/6, and PHABULOSA is required to maintain a stable bisymmetric pattern. We have verified this prediction by observing loss of bisymmetry in shr mutants. The model reveals the importance of several features of the network, namely the mutual degradation of microRNA165/6 and PHABULOSA and the existence of an additional negative regulator of cytokinin signaling. These components form a plausible mechanism capable of patterning vascular tissues in the absence of positional inputs provided by the transport of hormones from the shoot.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.31511
dc.identifier.eissn1091-6490
dc.identifier.issn0027-8424
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284140
dc.languageeng
dc.language.isoeng
dc.publisherProceedings of the National Academy of Sciences
dc.publisher.urlhttp://dx.doi.org/10.1073/pnas.1221766111
dc.rightsPublisher's own licence
dc.subjectmathematical modeling
dc.subjectplant development
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectHomeodomain Proteins
dc.subjectMicroRNAs
dc.subjectMicroscopy, Confocal
dc.subjectModels, Biological
dc.subjectPlant Growth Regulators
dc.subjectPlant Roots
dc.subjectPlant Vascular Bundle
dc.subjectSignal Transduction
dc.subjectTranscription Factors
dc.titleIntegration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots.
dc.typeArticle
prism.endingPage862
prism.issueIdentifier2
prism.publicationDate2014
prism.publicationNameProc Natl Acad Sci U S A
prism.startingPage857
prism.volume111
rioxxterms.licenseref.startdate2014-01
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1073/pnas.1221766111

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