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Control of meiotic crossover interference by a proteolytic chaperone network

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Kim, Heejin 
Kim, Jaeil 
Son, Namil 

Abstract

Meiosis is a specialized eukaryotic division that produces genetically diverse gametes for sexual reproduction. During meiosis, homologous chromosomes pair and undergo reciprocal exchanges, called crossovers, which recombine genetic variation. Meiotic crossovers are stringently controlled with at least one obligate exchange forming per chromosome pair, while closely-spaced crossovers are inhibited by interference. In Arabidopsis, crossover positions can be explained by a diffusion-mediated coarsening model, in which large, approximately evenly-spaced foci of the pro-crossover E3 ligase HEI10 grow at the expense of smaller, closely-spaced clusters. However, the mechanisms that control HEI10 dynamics during meiosis remain unclear. Here, through a forward genetic screen in Arabidopsis we identified high crossover rate3 (hcr3), a dominant-negative mutant that reduces crossover interference and increases crossovers genome-wide. HCR3 encodes J3, a co-chaperone related to HSP40, which acts to target protein aggregates and biomolecular condensates to the disassembly chaperone HSP70, thereby promoting proteasomal degradation. Consistently, we show that a network of HCR3 and HSP70 chaperones facilitates proteolysis of HEI10, thereby regulating interference and the recombination landscape. These results reveal a new role for the HSP40/J3-HSP70 chaperones in regulating chromosome-wide dynamics of recombination via control of HEI10 proteolysis.

Description

Keywords

Arabidopsis, Crossing Over, Genetic, Proteolysis, Arabidopsis Proteins, Meiosis

Journal Title

Nature Plants

Conference Name

Journal ISSN

2055-026X
2055-0278

Volume Title

Publisher

Springer Nature
Sponsorship
European Research Council (681987)
Biotechnology and Biological Sciences Research Council (BB/S006842/1)
Biotechnology and Biological Sciences Research Council (BB/S020012/1)
BBSRC (BB/V003984/1)
Biotechnology and Biological Sciences Research Council (BB/N007557/1)
ERC