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Integrated Pharmacodynamic Analysis Identifies Two Metabolic Adaption Pathways to Metformin in Breast Cancer.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Lord, Simon R 
Cheng, Wei-Chen 
Liu, Dan 
Gaude, Edoardo 
Haider, Syed 

Abstract

Late-phase clinical trials investigating metformin as a cancer therapy are underway. However, there remains controversy as to the mode of action of metformin in tumors at clinical doses. We conducted a clinical study integrating measurement of markers of systemic metabolism, dynamic FDG-PET-CT, transcriptomics, and metabolomics at paired time points to profile the bioactivity of metformin in primary breast cancer. We show metformin reduces the levels of mitochondrial metabolites, activates multiple mitochondrial metabolic pathways, and increases 18-FDG flux in tumors. Two tumor groups are identified with distinct metabolic responses, an OXPHOS transcriptional response (OTR) group for which there is an increase in OXPHOS gene transcription and an FDG response group with increased 18-FDG uptake. Increase in proliferation, as measured by a validated proliferation signature, suggested that patients in the OTR group were resistant to metformin treatment. We conclude that mitochondrial response to metformin in primary breast cancer may define anti-tumor effect.

Description

Keywords

breast neoplasms, cancer metabolism, clinical study, gene expression profiling, metabolomics, metformin, mitochondria, positron emission tomography, Adult, Aged, Antineoplastic Agents, Breast Neoplasms, Female, Gene Expression Regulation, Neoplastic, Glucose, Humans, Hypoglycemic Agents, Metabolic Networks and Pathways, Metformin, Middle Aged, Mitochondria, Positron Emission Tomography Computed Tomography, Transcriptome

Journal Title

Cell Metab

Conference Name

Journal ISSN

1550-4131
1932-7420

Volume Title

28

Publisher

Elsevier BV
Sponsorship
MRC (unknown)
Medical Research Council (MC_UU_12022/6)