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F-actin coordinates spindle morphology and function in Drosophila meiosis.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Shi, Xingzhu 

Abstract

Meiosis is a highly conserved feature of sexual reproduction that ensures germ cells have the correct number of chromosomes prior to fertilization. A subset of microtubules, known as the spindle, are essential for accurate chromosome segregation during meiosis. Building evidence in mammalian systems has recently highlighted the unexpected requirement of the actin cytoskeleton in chromosome segregation; a network of spindle actin filaments appear to regulate many aspects of this process. Here we show that Drosophila oocytes also have a spindle population of actin that appears to regulate the formation of the microtubule spindle and chromosomal movements throughout meiosis. We demonstrate that genetic and pharmacological disruption of the actin cytoskeleton has a significant impact on spindle morphology, dynamics, and chromosome alignment and segregation during maturation and the metaphase-anaphase transition. We further reveal a role for calcium in maintaining the microtubule spindle and spindle actin. Together, our data highlights potential conservation of morphology and mechanism of the spindle actin during meiosis.

Description

Keywords

3101 Biochemistry and Cell Biology, 3105 Genetics, 31 Biological Sciences, Contraception/Reproduction, Genetics, 1 Underpinning research, 1.1 Normal biological development and functioning, Generic health relevance

Journal Title

PLoS Genet

Conference Name

Journal ISSN

1553-7390
1553-7404

Volume Title

Publisher

Public Library of Science (PLoS)
Sponsorship
Wellcome Trust (200734/Z/16/Z)
BBSRC (1944029)