An Indocyanine GreenāBased Nanoprobe for In Vivo Detection of Cellular Senescence
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Peer-reviewed
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jats:titleAbstract</jats:title>jats:pThere is an urgent need to improve conventional cancerātreatments by preventing detrimental side effects, cancer recurrence and metastases. Recent studies have shown that presence of senescent cells in tissues treated with chemoā or radiotherapy can be used to predict the effectiveness of cancer treatment. However, although the accumulation of senescent cells is one of the hallmarks of cancer, surprisingly little progress has been made in development of strategies for their detection in vivo. To address a lack of detection tools, we developed a biocompatible, injectable organic nanoprobe (NanoJagg), which is selectively taken up by senescent cells and accumulates in the lysosomes. The NanoJagg probe is obtained by selfāassembly of indocyanine green (ICG) dimers using a scalable manufacturing process and characterized by a unique spectral signature suitable for both photoacoustic tomography (PAT) and fluorescence imaging. In vitro, ex vivo and in vivo studies all indicate that NanoJaggs are a clinically translatable probe for detection of senescence and their PAT signal makes them suitable for longitudinal monitoring of the senescence burden in solid tumors after chemotherapy or radiotherapy.</jats:p>
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Publication status: Published
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1521-3757
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Medical Research Council (MR/R000530/1)
Engineering and Physical Sciences Research Council (EP/R003599/1)
Engineering and Physical Sciences Research Council (EP/S009000/1)
Cancer Research UK (27887)
Cancer Research UK (C62187/A29760)