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Synthesis and import of GDP-l-fucose into the Golgi affect plant-water relations.

Published version
Peer-reviewed

Repository DOI


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Abstract

Land plants evolved multiple adaptations to restrict transpiration. However, the underlying molecular mechanisms are not sufficiently understood. We used an ozone-sensitivity forward genetics approach to identify Arabidopsis thaliana mutants impaired in gas exchange regulation. High water loss from detached leaves and impaired decrease of leaf conductance in response to multiple stomata-closing stimuli were identified in a mutant of MURUS1 (MUR1), an enzyme required for GDP-l-fucose biosynthesis. High water loss observed in mur1 was independent from stomatal movements and instead could be linked to metabolic defects. Plants defective in import of GDP-l-Fuc into the Golgi apparatus phenocopied the high water loss of mur1 mutants, linking this phenotype to Golgi-localized fucosylation events. However, impaired fucosylation of xyloglucan, N-linked glycans, and arabinogalactan proteins did not explain the aberrant water loss of mur1 mutants. Partial reversion of mur1 water loss phenotype by borate supplementation and high water loss observed in boron uptake mutants link mur1 gas exchange phenotypes to pleiotropic consequences of l-fucose and boron deficiency, which in turn affect mechanical and morphological properties of stomatal complexes and whole-plant physiology. Our work emphasizes the impact of fucose metabolism and boron uptake on plant-water relations.

Description

Publication status: Published


Funder: Ella ja Georg Ehrnroothin Säätiö; doi: http://dx.doi.org/10.13039/501100003502


Funder: European Regional Development Fund; doi: http://dx.doi.org/10.13039/501100008530


Funder: Helsingin Yliopisto; doi: http://dx.doi.org/10.13039/100007797


Funder: Opetushallitus; doi: http://dx.doi.org/10.13039/501100011842


Funder: Suomen Kulttuurirahasto; doi: http://dx.doi.org/10.13039/501100003125

Keywords

cell wall, fucose metabolism, plant-water relations, rhamnogalacturonan II, stomata, Fucose, Guanosine Diphosphate Fucose, Boron, Arabidopsis, Polysaccharides

Journal Title

New Phytol

Conference Name

Journal ISSN

1469-8137
1469-8137

Volume Title

Publisher

Wiley
Sponsorship
Academy of Finland (266793, 294580, 333703)
Eesti Teadusagentuur (PRG433, PRG719, PUT311)
FP7 Research infrastructures (EPPN grant no. 284443)