Following the assembly of functional circuitry: high resolution large-scale population neuronal dynamics in the neonatal mouse retina

Following the assembly of functional circuitry: high resolution large-scale population neuronal dynamics in the neonatal mouse retina

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http://www.dspace.cam.ac.uk/handle/1810/244335

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A short sequence of raw spontaneous activity recorded from a P11 retina in the presence of 10 µM bicuculline (same retina as in Figure 1 in the main paper). The activity is color-coded according to the minimum-maximum variations in voltage within 10 ms windows, with blue shades for no activity and warm color showing subthreshold events and spikes. This retina was particularly active, with repetitive waves flowing towards and away from the optic disc (non-active area in the middle of the field). The total episode real time is 33s. (1.04 MB)

Waves detected in a P4 retina. Wave detection is based on a combination of burst temporal overlap and spatial proximity criteria (see Methods for details). The activity is shown in 1 s bins. Each separate wave is in a different color with decreasing brightness indicating time. The black dots show channels on which bursts are detected at any time during the entire dataset (which is longer than this video clip) and the gray background represents all non-bursting (or non-active at all) channels on the array. The gray perimeter is the limit of the active area of the chip, measuring 2.67 mm x 2.67 mm. The left panel shows the activity detected at full resolution on the APS MEA and the right panel shows exactly the same dataset, but after downsampling the activity to an 8 x 8 array encompassing the same area as the APS MEA. Each electrode in this case covers 2x2 APS electrodes (42 µm x 42 µm) with a center-to-center separation of 240 µm (~ 6 APS channels). There is a mixture of small and large waves at that age, and the activity density is low. After downsampling it is still possible to see overall activity propagation across the RGC layer, but with significantly less accuracy. (6.63 MB)

Waves detected in a P6 retina. See legend of Movie 2 for more details. The striking difference between P4 and P6 is that here, at P6, the activity density is significantly higher, although this important difference is difficult to establish when comparing the low density recordings in the right panels, emphasizing the advantage of the high density system. (8.16 MB)

Waves detected in a P9 retina with the high resolution APS MEA. See legend of Movie 2 for more details. Most waves are relatively spatially restricted on the array. (7.26 MB)

Waves detected in the same P9 retina as in Movie 4, but following overnight incubation in 2 mM L-allylglycine, an inhibitor of glutamic acid decarboxylase, the enzyme responsible for GABA synthesis. L-allylglycine leads to depletion of endogenous GABA (see Chabrol et al., 2012). Waves are significantly larger and faster than in control conditions, demonstrating the important role of GABA in dampening wave propagation across the RGC layer. (8.15 MB)

Type

Authors

Maccione, Alessandro

Hennig, Matthias H

Gandolfo, Mauro

Muthmann, Oliver

Keywords

Neural Development

Publisher

TBC

Rights and licensing

All Rights Reserved

Except where otherwised noted, this item's license is described as All Rights Reserved

Sponsorship

This work was supported by BBSRC grant BB/H023569/1 (ES, MH, SJE, MD), MRC Fellowship G0900425 (MH), Istituto Italiano di Tecnologia (LB, AM, MG), EurosSPIN Erasmus Mundus programme (OM), EPSRC EP/E002331/1 and BBSRC BB/I001042/1 (CARMEN), Newcastle University Medical School (ES, JvC, AJ).

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Scholarly Works - Applied Mathematics and Theoretical Physics
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