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Maternally inherited piRNAs direct transient heterochromatin formation at active transposons during early Drosophila embryogenesis.

Accepted version
Peer-reviewed

Type

Article

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Authors

Falconio, Federica A 
Joud, Fadwa 
Lythgoe, Emily K 

Abstract

The PIWI-interacting RNA (piRNA) pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. In Drosophila, piRNAs are intergenerationally inherited through the maternal lineage, and this has demonstrated importance in the specification of piRNA source loci and in silencing of I- and P-elements in the germ cells of daughters. Maternally inherited Piwi protein enters somatic nuclei in early embryos prior to zygotic genome activation and persists therein for roughly half of the time required to complete embryonic development. To investigate the role of the piRNA pathway in the embryonic soma, we created a conditionally unstable Piwi protein. This enabled maternally deposited Piwi to be cleared from newly laid embryos within 30 min and well ahead of the activation of zygotic transcription. Examination of RNA and protein profiles over time, and correlation with patterns of H3K9me3 deposition, suggests a role for maternally deposited Piwi in attenuating zygotic transposon expression in somatic cells of the developing embryo. In particular, robust deposition of piRNAs targeting roo, an element whose expression is mainly restricted to embryonic development, results in the deposition of transient heterochromatic marks at active roo insertions. We hypothesize that roo, an extremely successful mobile element, may have adopted a lifestyle of expression in the embryonic soma to evade silencing in germ cells.

Description

Keywords

D. melanogaster, Drosophila, chromatin, chromosomes, developmental biology, embryogenesis, epigenetics, gene expression, piRNA pathway, transposable elements, Animals, Chromatin, DNA Transposable Elements, Developmental Biology, Drosophila, Drosophila Proteins, Drosophila melanogaster, Embryonic Development, Epigenomics, Female, Gene Expression, Germ Cells, Heterochromatin, Histones, Male, Maternal Inheritance, RNA, Small Interfering

Journal Title

Elife

Conference Name

Journal ISSN

2050-084X
2050-084X

Volume Title

10

Publisher

eLife Sciences Publications, Ltd

Rights

All rights reserved
Sponsorship
Royal Society (RP130039)
Wellcome Trust (110161/Z/15/Z)
Cancer Research UK (C14303/A17197)
Cancer Research UK (C14303/A17197)
Cancer Research UK (C9545/A29580_do not transfer)
Royal Society (RSRP\R\200001)
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