Repository logo
 

Chemical Annealing Restructures RNA for Nanopore Detection.

Accepted version
Peer-reviewed

Change log

Authors

Earle, Max K 
Keyser, Ulrich F 

Abstract

RNA is a key biochemical marker, yet its chemical instability and complex secondary structure hamper its integration into DNA nanotechnology-based sensing platforms. Relying on the denaturation of the native RNA structure using urea, we show that restructured DNA/RNA hybrids can readily be prepared at room temperature. Using solid-state nanopore sensing, we demonstrate that the structures of our DNA/RNA hybrids conform to the design at the single-molecule level. Employing this chemical annealing procedure, we mitigate RNA self-cleavage, enabling the direct detection of restructured RNA molecules for biosensing applications.

Description

Keywords

34 Chemical Sciences, Genetics, Nanotechnology, Bioengineering

Journal Title

J Am Chem Soc

Conference Name

Journal ISSN

0002-7863
1520-5126

Volume Title

Publisher

American Chemical Society (ACS)
Sponsorship
European Commission Horizon 2020 (H2020) Future and Emerging Technologies (FET) (964995)
Herchel Smith Fellowship, European Union under the Horizon 2020 Program, FET- Open: DNA-FAIRYLIGHTS, Grant Agreement No. 964995, UK Research and Innovation (UKRI) under the UK government’s Horizon Europe funding guarantee EP/X023311/1.