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Generation of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation.

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cam.issuedOnline2018-09-20
dc.contributor.authorHamanaka, Sanae
dc.contributor.authorUmino, Ayumi
dc.contributor.authorSato, Hideyuki
dc.contributor.authorHayama, Tomonari
dc.contributor.authorYanagida, Ayaka
dc.contributor.authorMizuno, Naoaki
dc.contributor.authorKobayashi, Toshihiro
dc.contributor.authorKasai, Mariko
dc.contributor.authorSuchy, Fabian Patrik
dc.contributor.authorYamazaki, Satoshi
dc.contributor.authorMasaki, Hideki
dc.contributor.authorYamaguchi, Tomoyuki
dc.contributor.authorNakauchi, Hiromitsu
dc.contributor.orcidYanagida, Ayaka [0000-0002-6350-2185]
dc.date.accessioned2018-12-14T00:31:58Z
dc.date.available2018-12-14T00:31:58Z
dc.date.issued2018-10-09
dc.description.abstractIn the case of organ transplantation accompanied by vascular anastomosis, major histocompatibility complex mismatched vascular endothelial cells become a target for graft rejection. Production of a rejection-free, transplantable organ, therefore, requires simultaneous generation of vascular endothelial cells within the organ. To generate pluripotent stem cell (PSC)-derived vascular endothelial cells, we performed blastocyst complementation with a vascular endothelial growth factor receptor-2 homozygous mutant blastocyst. This mutation is embryonic lethal at embryonic (E) day 8.5-9.5 due to an early defect in endothelial and hematopoietic cells. The Flk-1 homozygous knockout chimeric mice survived to adulthood for over 1 year without any abnormality, and all vascular endothelial cells and hematopoietic cells were derived from the injected PSCs. This approach could be used in conjunction with other gene knockouts which induce organ deficiency to produce a rejection-free, transplantable organ in which all the organ's cells and vasculature are PSC derived.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.34249
dc.identifier.eissn2213-6711
dc.identifier.issn2213-6711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286940
dc.languageeng
dc.language.isoeng
dc.publisherElsevier BV
dc.publisher.urlhttp://dx.doi.org/10.1016/j.stemcr.2018.08.015
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectblastocyst complementation
dc.subjecthematopoietic cells
dc.subjectpluripotent stem cells
dc.subjecttissue regeneration
dc.subjectvascular endothelial cells
dc.subjectAging
dc.subjectAnimals
dc.subjectBlastocyst
dc.subjectChimera
dc.subjectEndothelial Cells
dc.subjectHematopoietic Stem Cells
dc.subjectInduced Pluripotent Stem Cells
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectNeovascularization, Physiologic
dc.subjectPericytes
dc.subjectPhenotype
dc.subjectPlatelet Endothelial Cell Adhesion Molecule-1
dc.subjectVascular Endothelial Growth Factor Receptor-2
dc.titleGeneration of Vascular Endothelial Cells and Hematopoietic Cells by Blastocyst Complementation.
dc.typeArticle
dcterms.dateAccepted2018-08-22
prism.endingPage997
prism.issueIdentifier4
prism.publicationDate2018
prism.publicationNameStem Cell Reports
prism.startingPage988
prism.volume11
rioxxterms.licenseref.startdate2018-10
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1016/j.stemcr.2018.08.015

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