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Detecting eukaryotic microbiota with single-cell sensitivity in human tissue.

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cam.issuedOnline2018-09
dc.contributor.authorLager, Susanne
dc.contributor.authorde Goffau, Marcus C
dc.contributor.authorSovio, Ulla
dc.contributor.authorPeacock, Sharon J
dc.contributor.authorParkhill, Julian
dc.contributor.authorCharnock-Jones, D Stephen
dc.contributor.authorSmith, Gordon CS
dc.contributor.orcidLager, Susanne [0000-0003-3556-065X]
dc.date.accessioned2018-10-03T04:44:57Z
dc.date.available2018-10-03T04:44:57Z
dc.date.issued2018-09-01
dc.description.abstractBACKGROUND: Fetal growth restriction, pre-eclampsia, and pre-term birth are major adverse pregnancy outcomes. These complications are considerable contributors to fetal/maternal morbidity and mortality worldwide. A significant proportion of these cases are thought to be due to dysfunction of the placenta. However, the underlying mechanisms of placental dysfunction are unclear. The aim of the present study was to investigate whether adverse pregnancy outcomes are associated with evidence of placental eukaryotic infection. RESULTS: We modified the 18S Illumina Amplicon Protocol of the Earth Microbiome Project and made it capable of detecting just a single spiked-in genome copy of Plasmodium falciparum, Saccharomyces cerevisiae, or Toxoplasma gondii among more than 70,000 human cells. Using this method, we were unable to detect eukaryotic pathogens in placental biopsies in instances of adverse pregnancy outcome (n = 199) or in healthy controls (n = 99). CONCLUSIONS: Eukaryotic infection of the placenta is not an underlying cause of the aforementioned pregnancy complications. Possible clinical applications for this non-targeted, yet extremely sensitive, eukaryotic screening method are manifest.
dc.description.sponsorshipNational Institute for Health Research (NIHR) Cambridge Biomedical Research Centre (Women’s Health theme) Wellcome Trust Sanger Institute
dc.format.mediumElectronic
dc.identifier.doi10.17863/CAM.30438
dc.identifier.eissn2049-2618
dc.identifier.issn2049-2618
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283076
dc.languageeng
dc.language.isoeng
dc.publisherSpringer Science and Business Media LLC
dc.publisher.urlhttp://dx.doi.org/10.1186/s40168-018-0529-x
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject18S rRNA gene
dc.subjectFetal growth restriction
dc.subjectInfection
dc.subjectPlacenta
dc.subjectPre-eclampsia
dc.subjectPre-term birth
dc.subjectPregnancy complication
dc.subjectSequencing
dc.subjectAdult
dc.subjectEukaryota
dc.subjectFemale
dc.subjectHumans
dc.subjectPlacenta
dc.subjectPlasmodium falciparum
dc.subjectPregnancy
dc.subjectPregnancy Complications
dc.subjectPregnancy Outcome
dc.subjectSaccharomyces cerevisiae
dc.subjectToxoplasma
dc.subjectYoung Adult
dc.titleDetecting eukaryotic microbiota with single-cell sensitivity in human tissue.
dc.typeArticle
dcterms.dateAccepted2018-08-09
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameMicrobiome
prism.startingPage151
prism.volume6
pubs.funder-project-idMedical Research Council (MR/K021133/1)
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (146281)
pubs.funder-project-idMedical Research Council (G1100221)
pubs.funder-project-idMedical Research Council (G1100221/1)
rioxxterms.licenseref.startdate2018-09
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1186/s40168-018-0529-x

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