Collagen labelling with an azide-proline chemical reporter in live cells.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Amgarten, Beatrice
Martínez-Sáez, Nuria
Oliveira, Bruno L
Albuquerque, Inês S
Abstract
We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.
Description
Keywords
Alkynes, Azides, Cell Survival, Cells, Cultured, Collagen, Cyclization, Humans, Osteoblasts, Proline
Journal Title
Chem Commun (Camb)
Conference Name
Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
51
Publisher
Royal Society of Chemistry (RSC)
Publisher DOI
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/G021392/1)
Engineering and Physical Sciences Research Council (EP/M003647/1)
Engineering and Physical Sciences Research Council (EP/M003647/1)