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Microelectrode Arrays Measure Blocking of Voltage-Gated Calcium Ion Channels on Supported Lipid Bilayers Derived from Primary Neurons.

Published version
Peer-reviewed

Repository DOI


Change log

Authors

Fernandez-Villegas, Ana 
Withers, Aimee 
Wheeler, Alexandra 

Abstract

Drug studies targeting neuronal ion channels are crucial to understand neuronal function and develop therapies for neurological diseases. The traditional method to study neuronal ion-channel activities heavily relies on the whole-cell patch clamp as the industry standard. However, this technique is both technically challenging and labour-intensive, while involving the complexity of keeping cells alive with low throughput. Therefore, the shortcomings are limiting the efficiency of ion-channel-related neuroscience research and drug testing. Here, this work reports a new system of integrating neuron membranes with organic microelectrode arrays (OMEAs) for ion-channel-related drug studies. This work demonstrates that the supported lipid bilayers (SLBs) derived from both neuron-like (neuroblastoma) cells and primary neurons are integrated with OMEAs for the first time. The increased expression of voltage-gated calcium (CaV) ion channels on differentiated SH-SY5Y SLBs  compared to non-differentiated ones is sensed electrically. Also, dose-response of the CaV ion-channel blocking effect on primary cortical neuronal SLBs from rats is monitored. The dose range causing ion channel blocking is comparable to literature. This system overcomes the major challenges from traditional methods (e.g., patch clamp) and showcases an easy-to-test, rapid, ultra-sensitive, cell-free, and high-throughput platform to monitor dose-dependent ion-channel blocking effects on native neuronal membranes.

Description

Publication status: Published


Funder: Engineering and Physical Sciences Research Council Centre for Doctoral Training in Sensor Technologies and Applications

Keywords

PEDOT:PSS, blebbing, microelectrode arrays, neurons, supported lipid bilayers, voltage-gated ion channels

Journal Title

Adv Sci (Weinh)

Conference Name

Journal ISSN

2198-3844
2198-3844

Volume Title

Publisher

Wiley
Sponsorship
Engineering and Physical Sciences Research Council (EP/L015889/1)
European Research Council (723951)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (895801)
United States Air Force (FA86552017021)