Chromosomal Signatures Corroborate the Phylogenetic Relationships within Akodontini (Rodentia, Sigmodontinae).


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Article
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Authors
Oliveira da Silva, Willam  ORCID logo  https://orcid.org/0000-0003-3125-1075
Malcher, Stella Miranda 
Pereira, Adenilson Leão  ORCID logo  https://orcid.org/0000-0003-2687-5297
Pieczarka, Julio Cesar 
Ferguson-Smith, Malcolm Andrew 
Abstract

Comparative chromosome-painting analysis among highly rearranged karyotypes of Sigmodontinae rodents (Rodentia, Cricetidae) detects conserved syntenic blocks, which are proposed as chromosomal signatures and can be used as phylogenetic markers. In the Akodontini tribe, the molecular topology (Cytb and/or IRBP) shows five low-supported clades (divisions: "Akodon", "Bibimys", "Blarinomys", "Oxymycterus", and "Scapteromys") within two high-supported major clades (clade A: "Akodon", "Bibimys", and "Oxymycterus"; clade B: "Blarinomys" and "Scapteromys"). Here, we examine the chromosomal signatures of the Akodontini tribe by using Hylaeamys megacephalus (HME) probes to study the karyotypes of Oxymycterus amazonicus (2n = 54, FN = 64) and Blarinomys breviceps (2n = 28, FN = 50), and compare these data with those from other taxa investigated using the same set of probes. We strategically employ the chromosomal signatures to elucidate phylogenetic relationships among the Akodontini. When we follow the evolution of chromosomal signature states, we find that the cytogenetic data corroborate the current molecular relationships in clade A nodes. We discuss the distinct events that caused karyotypic variability in the Oxymycterus and Blarinomys genera. In addition, we propose that Blarinomys may constitute a species complex, and that the taxonomy should be revised to better delimit the geographical boundaries and their taxonomic status.

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Keywords
FISH, chromosome painting, conserved syntenic block, rodents, Animals, Biological Evolution, Brazil, Chromosome Painting, Cytogenetics, Geography, Karyotype, Karyotyping, Male, Phylogeny, Rodentia, Sigmodontinae, Synteny
Journal Title
Int J Mol Sci
Conference Name
Journal ISSN
1661-6596
1422-0067
Volume Title
21
Publisher
MDPI AG