Structural basis for stabilisation of the RAD51 nucleoprotein filament by BRCA2.

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Joudeh, Luay 
Cobbett, Katie 

The BRCA2 tumour suppressor protein preserves genomic integrity via interactions with the DNA-strand exchange RAD51 protein in homology-directed repair. The RAD51-binding TR2 motif at the BRCA2 C-terminus is essential for protection and restart of stalled replication forks. Biochemical evidence shows that TR2 recognises filamentous RAD51, but existing models of TR2 binding to RAD51 lack a structural basis. Here we used cryo-electron microscopy and structure-guided mutagenesis to elucidate the mechanism of TR2 binding to nucleoprotein filaments of human RAD51. We find that TR2 binds across the protomer interface in the filament, acting as a brace for adjacent RAD51 molecules. TR2 targets an acidic-patch motif on human RAD51 that serves as a recruitment hub in fission yeast Rad51 for recombination mediators Rad52 and Rad55-Rad57. Our findings provide a structural rationale for RAD51 filament stabilisation by BRCA2 and reveal a common recruitment mechanism of recombination mediators to the RAD51 filament.

Humans, DNA-Binding Proteins, Nucleoproteins, Cryoelectron Microscopy, Rad51 Recombinase, BRCA2 Protein, DNA Repair
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Nat Commun
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Nature Portfolio
Wellcome Trust (221892/Z/20/Z)
Cambridge BBSRC DTP,
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