Structural Determination of the Australian Bat Lyssavirus Nucleoprotein and Phosphoprotein Complex.

Published version
Repository DOI

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Authors
Donnelly, Camilla M  ORCID logo  https://orcid.org/0000-0003-2098-2462
Stewart, Murray 
Sundaramoorthy, Vinod  ORCID logo  https://orcid.org/0000-0001-6309-8031
Forwood, Jade K 
Abstract

Australian bat lyssavirus (ABLV) shows similar clinical symptoms as rabies, but there are currently no protein structures available for ABLV proteins. In lyssaviruses, the interaction between nucleoprotein (N) and phosphoprotein (N) in the absence of RNA generates a complex (N0P) that is crucial for viral assembly, and understanding the interface between these two proteins has the potential to provide insight into a key feature: the viral lifecycle. In this study, we used recombinant chimeric protein expression and X-ray crystallography to determine the structure of ABLV nucleoprotein bound to residues 1-40 of its phosphoprotein chaperone. Comparison of our results with the recently generated structure of RABV CVS-11 N0P demonstrated a highly conserved interface in this complex. Because the N0P interface is conserved in the lyssaviruses of phylogroup I, it is an attractive therapeutic target for multiple rabies-causing viral species.

Description

Peer reviewed: True


Acknowledgements: This research was undertaken in part using the MX2 beamline at the Australian Synchrotron, part of ANSTO, and made use of the Australian Cancer Research Foundation (ACRF) detector. J.K.F., J.A.R. and C.M.D. acknowledge support from the Training Hub promoting Regional Industry and Innovation in Virology and Epidemiology (THRIIVE).


Publication status: Published


Funder: Gulbali Graduate Bridging Scheme

Keywords
Australian bat lyssavirus, X-ray crystallography, nucleocapsid, nucleoprotein, phosphoprotein, rabies virus, rhabdovirus, small-angle X-ray scattering, Animals, Lyssavirus, Rabies, Nucleoproteins, Chiroptera, Australia, Phosphoproteins, Rhabdoviridae Infections
Journal Title
Viruses
Conference Name
Journal ISSN
1999-4915
1999-4915
Volume Title
16
Publisher
MDPI AG
Sponsorship
MRC (MC-A025-5PL41)
Australian Government (DE210101145)