Analysis of patchclamp recordings: model-free multiscale methods and software.


Change log
Authors
Eltzner, Benjamin 
Munk, Axel 
Abstract

Analysis of patchclamp recordings is often a challenging issue. We give practical guidance how such recordings can be analyzed using the model-free multiscale idealization methodology JSMURF, JULES, and HILDE. We provide an operational manual how to use the accompanying software available as an R-package and as a graphical user interface. This includes selection of the right approach and tuning of parameters. We also discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other.

Description
Keywords
Deconvolution, Flickering, Fully automatic, Hidden Markov models, Homogeneous and heterogeneous noise, Ion channel recordings, Low-pass filtering, Open-channel noise, PorB, Subconductance states, Algorithms, Ion Channels, Kinetics, Markov Chains, Patch-Clamp Techniques, Software
Journal Title
Eur Biophys J
Conference Name
Journal ISSN
0175-7571
1432-1017
Volume Title
50
Publisher
Springer Science and Business Media LLC
Sponsorship
Engineering and Physical Sciences Research Council (EP/N031938/1)