Repository logo

Regulation of chemotropic guidance of nerve growth cones by microRNA.

Change log


Han, Liang 
Wen, Zhexing 
Lynn, Rachel C 
Baudet, Marie-Laure 
Holt, Christine E 


BACKGROUND: The small non-coding microRNAs play an important role in development by regulating protein translation, but their involvement in axon guidance is unknown. Here, we investigated the role of microRNA-134 (miR-134) in chemotropic guidance of nerve growth cones. RESULTS: We found that miR-134 is highly expressed in the neural tube of Xenopus embryos. Fluorescent in situ hybridization also showed that miR-134 is enriched in the growth cones of Xenopus spinal neurons in culture. Importantly, overexpression of miR-134 mimics or antisense inhibitors blocked protein synthesis (PS)-dependent attractive responses of Xenopus growth cones to a gradient of brain-derived neurotrophic factor (BDNF). However, miR-134 mimics or inhibitors had no effect on PS-independent bidirectional responses of Xenopus growth cones to bone morphogenic protein 7 (BMP7). Our data further showed that Xenopus LIM kinase 1 (Xlimk1) mRNA is a potential target of miR-134 regulation. CONCLUSIONS: These findings demonstrate a role for miR-134 in translation-dependent guidance of nerve growth cones. Different guidance cues may act through distinct signaling pathways to elicit PS-dependent and -independent mechanisms to steer growth cones in response to a wide array of spatiotemporal cues during development.



Animals, Brain-Derived Neurotrophic Factor, Chemotaxis, Fluorescent Antibody Technique, Gene Expression Regulation, Growth Cones, Humans, MicroRNAs, Mitogen-Activated Protein Kinase 1, Models, Biological, Oligonucleotides, Antisense, Phosphorylation, Xenopus, Xenopus Proteins

Journal Title

Mol Brain

Conference Name

Journal ISSN


Volume Title


Springer Science and Business Media LLC
Wellcome Trust (085314/Z/08/Z)