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Intelectin-1 binds and alters the localization of Akkermansia muciniphila that modify the mucus barrier

Published version
Peer-reviewed

Type

Article

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Authors

Matute, Juan D 
Duan, Jinzhi 
Flak, Magdalena B 
Griebel, Paul 
Tascon-Arcila, Jose 

Abstract

Intelectin-1 (ITLN1) is a lectin secreted by intestinal epithelial cells (IECs) and upregulated in human ulcerative colitis (UC). We investigated how ITLN1 production is regulated in IECs, and the biological effects of ITLN1 at the host-microbiota interface using mouse models. Our data show that ITLN1 upregulation in IECs from UC patients is a consequence of activating the unfolded protein response. Analysis of microbes coated by ITLN1 in vivo revealed a restricted subset of microorganisms, including the mucolytic bacterium Akkermansia muciniphila. Mice overexpressing intestinal ITLN1 exhibited decreased inner colonic mucus layer thickness and closer apposition of A. muciniphila to the epithelial cell surface, similar to alterations reported in UC. The changes in the inner mucus layer were microbiota- and A. muciniphila-dependent and associated with enhanced sensitivity to chemically-induced and T cell-mediated colitis. We conclude that, by determining the localization of a select group of bacteria to the mucus layer, ITLN1 modifies this critical barrier. Together these findings may explain the impact of ITLN1 dysregulation on UC pathogenesis.

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Keywords

Journal Title

Journal of Experimental Medicine

Conference Name

Journal ISSN

0022-1007

Volume Title

Publisher

Rockefeller University Press

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Sponsorship
Wellcome Trust (106260/Z/14/Z)
European Research Council (648889)
NIH, ERC, DFG