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Composition of Transcription Machinery and Its Crosstalk with Nucleoid-Associated Proteins and Global Transcription Factors.

Published version
Peer-reviewed

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Authors

Muskhelishvili, Georgi 
Sobetzko, Patrick 
Mehandziska, Sanja 
Travers, Andrew 

Abstract

The coordination of bacterial genomic transcription involves an intricate network of interdependent genes encoding nucleoid-associated proteins (NAPs), DNA topoisomerases, RNA polymerase subunits and modulators of transcription machinery. The central element of this homeostatic regulatory system, integrating the information on cellular physiological state and producing a corresponding transcriptional response, is the multi-subunit RNA polymerase (RNAP) holoenzyme. In this review article, we argue that recent observations revealing DNA topoisomerases and metabolic enzymes associated with RNAP supramolecular complex support the notion of structural coupling between transcription machinery, DNA topology and cellular metabolism as a fundamental device coordinating the spatiotemporal genomic transcription. We analyse the impacts of various combinations of RNAP holoenzymes and global transcriptional regulators such as abundant NAPs, on genomic transcription from this viewpoint, monitoring the spatiotemporal patterns of couplons-overlapping subsets of the regulons of NAPs and RNAP sigma factors. We show that the temporal expression of regulons is by and large, correlated with that of cognate regulatory genes, whereas both the spatial organization and temporal expression of couplons is distinctly impacted by the regulons of NAPs and sigma factors. We propose that the coordination of the growth phase-dependent concentration gradients of global regulators with chromosome configurational dynamics determines the spatiotemporal patterns of genomic expression.

Description

Keywords

DNA topology, RNA polymerase supramolecular complex, metabolic enzymes, nucleoid-associated proteins, regulons and couplons, Bacteria, Bacterial Proteins, DNA, Bacterial, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, Transcription Factors, Transcription, Genetic

Journal Title

Biomolecules

Conference Name

Journal ISSN

2218-273X
2218-273X

Volume Title

11

Publisher

MDPI AG