Repository logo
 

The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum.

Simple item page
cam.issuedOnline2021-12-20
datacite.contributor.supervisoreditor: Dang, Weiwei
datacite.contributor.supervisorsenior_editor: Struhl, Kevin
dc.contributor.authorZhang, Ning
dc.contributor.authorSong, Limin
dc.contributor.authorXu, Yang
dc.contributor.authorPei, Xueyuan
dc.contributor.authorLuisi, Ben
dc.contributor.authorLiang, Wenxing
dc.contributor.orcidZhang, Ning [0000-0002-4824-3795]
dc.contributor.orcidLuisi, Ben [0000-0003-1144-9877]
dc.contributor.orcidLiang, Wenxing [0000-0002-3791-4901]
dc.date.accessioned2022-01-10T12:48:24Z
dc.date.available2022-01-10T12:48:24Z
dc.date.issued2021-12-20
dc.date.submitted2021-11-15
dc.date.updated2022-01-10T12:48:23Z
dc.description.abstractFusarium oxysporum is one of the most important pathogenic fungi with a broad range of plant and animal hosts. The first key step of its infection cycle is conidial germination, but there is limited information available on the molecular events supporting this process. We show here that germination is accompanied by a sharp decrease in expression of FoSir5, an ortholog of the human lysine deacetylase SIRT5. We observe that FoSir5 decrotonylates a subunit of the fungal pyruvate dehydrogenase complex (FoDLAT) at K148, resulting in inhibition of the activity of the complex in mitochondria. Moreover, FoSir5 decrotonylates histone H3K18, leading to a downregulation of transcripts encoding enzymes of aerobic respiration pathways. Thus, the activity of FoSir5 coordinates regulation in different organelles to steer metabolic flux through respiration. As ATP content is positively related to fungal germination, we propose that FoSir5 negatively modulates conidial germination in F. oxysporum through its metabolic impact. These findings provide insights into the multifaceted roles of decrotonylation, catalyzed by FoSir5, that support conidial germination in F. oxysporum.
dc.identifier.doi10.17863/CAM.79976
dc.identifier.eissn2050-084X
dc.identifier.issn2050-084X
dc.identifier.other75583
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332526
dc.languageen
dc.publishereLife Sciences Publications, Ltd
dc.subjectResearch Article
dc.subjectMicrobiology and Infectious Disease
dc.subjectPlant Biology
dc.subjectconidial germinaiton
dc.subjectlysine crotonylation
dc.subjectphytopathogenic fungi
dc.subjectATP metabolism
dc.subjectOther
dc.titleThe decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination of Fusarium oxysporum.
dc.typeArticle
dcterms.dateAccepted2021-12-15
prism.publicationNameElife
prism.volume10
pubs.funder-project-idWellcome Trust (200873/Z/16/Z)
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.7554/eLife.75583

Files

Original bundle
Now showing 1 - 4 of 4
Thumbnail Image
Name:
elife-75583.pdf
Size:
3.67 MB
Format:
Adobe Portable Document Format
Description:
Published version
Licence
http://creativecommons.org/licenses/by/4.0/
Download
No Thumbnail Available
Name:
additional-files.zip
Size:
26.19 MB
Format:
ZIP file
Description:
Supporting information
Licence
http://creativecommons.org/licenses/by/4.0/
Download
Thumbnail Image
Name:
elife-75583-figures.pdf
Size:
9.59 MB
Format:
Adobe Portable Document Format
Description:
Published version
Licence
http://creativecommons.org/licenses/by/4.0/
Download
No Thumbnail Available
Name:
elife-75583.xml
Size:
170.12 KB
Format:
Extensible Markup Language
Description:
Bibliographic metadata
Licence
http://creativecommons.org/licenses/by/4.0/
Download

Collections

Jisc Publications Router