Repository logo
 

Imaging the Effector CD8 Synapse.

Accepted version
Peer-reviewed

Repository URI

https://www.repository.cam.ac.uk/handle/1810/279509

Repository DOI

https://doi.org/10.17863/CAM.26883
Thumbnail Image

Files

Accepted version (24.24 MB)

Type

Article

Change log

Authors

Abstract

Here, we describe 4D imaging of effector CD8+ T cells as they conjugate and kill live targets in vitro and analyze the polarization dynamics of intracellular compartments to this cell-cell interface.

Description

Keywords

4D object-based image analysis, Imaris, Nucleofection, Time-lapse confocal imaging, Animals, CD8-Positive T-Lymphocytes, Humans, Imaging, Three-Dimensional, Immunological Synapses, Microscopy, Confocal

Journal Title

Methods Mol Biol

Conference Name

Journal ISSN

1064-3745
1940-6029

Volume Title

1584

Publisher

Springer Nature

Publisher DOI

https://doi.org/10.1007/978-1-4939-6881-7_29

Rights and licensing

undefined
Except where otherwised noted, this item's license is described as http://www.rioxx.net/licenses/all-rights-reserved
Sponsorship
Wellcome Trust (103930/Z/14/Z)

Collections

University of Cambridge Research Outputs (Articles and Conferences)