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An alternative mode of epithelial polarity in the Drosophila midgut

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cam.issuedOnline2018-10-19
datacite.isderivedfrom.doi10.1101/307579
dc.contributor.authorChen, Jia
dc.contributor.authorSayadian, Aram-Christopher
dc.contributor.authorLowe, Nick
dc.contributor.authorLovegrove, Holly
dc.contributor.authorSt Johnston, Daniel
dc.contributor.orcidChen, Jia [0000-0003-0080-0748]
dc.contributor.orcidSt Johnston, Daniel [0000-0001-5582-3301]
dc.date.accessioned2019-01-31T10:32:25Z
dc.date.accessioned2019-02-02T00:31:52Z
dc.date.available2019-01-31T10:32:25Z
dc.date.available2019-02-02T00:31:52Z
dc.date.issued2018-10
dc.description.abstractApical-basal polarity is essential for the formation and function of epithelial tissues, whereas loss of polarity is a hallmark of tumours. Studies in Drosophila have identified conserved polarity factors that define the apical (Crumbs, Stardust, Par-6, aPKC), junctional (Baz/Par-3) and basolateral (Scribbled, Discs large, Lgl) domains of epithelial cells1. Because these conserved factors mark equivalent domains in diverse vertebrate and invertebrate epithelial types, it is generally assumed that this system organises polarity in all epithelia. Here we show that this is not the case, as none of these canonical factors are required for the polarisation of the endodermal epithelium of the Drosophila adult midgut. Furthermore, unlike other Drosophila epithelia, the midgut forms occluding junctions above adherens junctions, as in vertebrates, and requires the integrin adhesion complex for polarity. Thus, Drosophila contains two types of epithelia that polarise by different mechanisms. Since knock-outs of canonical polarity factors often have little effect on the polarity of vertebrate epithelia, this diversity of polarity mechanisms is likely to be conserved in other animals.
dc.identifier.doi10.17863/CAM.35997
dc.identifier.eissn1545-7885
dc.identifier.issn1544-9173
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288737
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.publisher.urlhttps://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3000041
dc.relation.replaceshttps://www.repository.cam.ac.uk/handle/1810/288609
dc.relation.replaces1810/288609
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectBody Patterning
dc.subjectCell Polarity
dc.subjectDigestive System
dc.subjectDrosophila Proteins
dc.subjectDrosophila melanogaster
dc.subjectEpithelial Cells
dc.subjectEpithelium
dc.subjectFemale
dc.subjectGenes, Insect
dc.subjectIntercellular Junctions
dc.subjectModels, Biological
dc.titleAn alternative mode of epithelial polarity in the Drosophila midgut
dc.typeArticle
dcterms.dateAccepted2018-10-02
prism.issueIdentifier10
prism.numbere3000041
prism.publicationNamePLoS Biology
prism.volume16
pubs.funder-project-idWellcome Trust (080007/B/06/Z)
pubs.funder-project-idWellcome Trust (080007/Z/06/Z)
pubs.funder-project-idWellcome Trust (207496/Z/17/Z)
pubs.funder-project-idCancer Research Uk (None)
pubs.funder-project-idWellcome Trust (203144/Z/16/Z)
pubs.funder-project-idCancer Research UK (C6946/A24843)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)
pubs.funder-project-idWellcome Trust (083348/Z/07/Z)
rioxxterms.licenseref.startdate2018-10-02
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1371/journal.pbio.3000041

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