This directory contains all the original data from the publication "High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data".

Title: Research data supporting High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data Authors: Henrik Åhl, Yi Zhang, Henrik Jönsson Contact: henrik.jonsson@slcu.cam.ac.uk Date of data collection: 2017-2021 Associated DOI: 10.3389/fpls.2022.827147 Location: Sainsbury Laboratory, University of Cambridge Keywords: pin3, pin4, pin7, pin3pin4, pin3pin7, pin4pin7, pin3pin4pin7, aux1, aux1lax1lax2lax3, abcb1, abcb19, abcb1abcb19, pCLV3:dsRed x pPIN1:PIN1-GFP x myr-YFP, xxt1xxt2, xxt1xxt2xxt5, qua1, bag, cesa3(eli1), cesa6(prc1-1), csi1-3, det3, cesa1(any1), gabi, cu4, cmu1cmu2, cesa3(je5), act2-5, arabidopsis thaliana, shoot apical meristem, confocal, isosurface, phyllotaxis, apex identification, tissue segmentation, plant development, flower development, 3D phenotyping, high-throughput Microscopes: LSM880, LSM700, LSM780 Formats: .csv, .txt, .lsm, .tif

This dataset contains 3D image stacks of the following:

• Col-0 plant shoots
• pin3, pin4, pin7, pin3,4, pin3,7, pin4,7, pin3,4,7 mutant plants
• aux1, aux1lax1,2,3 mutant plant shoots
• abcb1, abcb19, abcb1,19 mutant plant shoots
• pCLV3::dsRed x pPIN1:PIN1-GFP x myr-YFP expressing plant shoots
• xxt1xxt2, xxt1xxt2xxt5, qua1*, bag*, cesa3(eli1), cesa6(prc1-1), csi1-3*, det3*, cesa1(any1), gabi*, cu4*, cmu1cmu2*, cesa3(je5), act2-5* mutant plant shoots (*: not used in the analysis)
• One pABCB:ABCB19-GFP expressing plant
• One Col-0 protoplast
• One Col-0 apical hook

The dataset also contains the output results from the analysis pipeline presented in the affiliated publication, as well as corresponding file metadata.

Code and scripts used for data generation and statistical analysis, including visualisation can be found at https://gitlab.com/slcu/teamHJ/publications/aahl_etal_2022.