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Artefactual nanoparticle activation of the inflammasome platform: in vitro evidence with a nano-formed calcium phosphate.

Accepted version
Peer-reviewed

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Authors

Pele, Laetitia 
Haas, Carolin T 
Faria, Nuno 
Brown, Andy 

Abstract

AIM: To determine whether in vitro experimental conditions dictate cellular activation of the inflammasome by apatitic calcium phosphate nanoparticles. MATERIAL & METHODS: The responses of blood-derived primary human cells to in situ-formed apatite were investigated under different experimental conditions to assess the effect of aseptic culture, cell rest and duration of particle exposure. Cell death and particle uptake were assessed, while IL-1β and caspase 1 responses, with and without lipopolysaccharide prestimulation, were evaluated as markers of inflammasome activation. RESULTS: Under carefully addressed experimental conditions, apatitic nanoparticles did not induce cell death or engage the inflammasome platform, although both could be triggered through artefacts of experimentation. CONCLUSION: In vitro studies often predict that engineered nanoparticles, such as synthetic apatite, are candidates for inflammasome activation and, hence, are toxic. However, the experimental setting must be very carefully considered as it may promote false-positive outcomes.

Description

Keywords

IL-1β, apatite, caspase 1, experimental conditions, inflammasome, nanoparticle, Apatites, Artifacts, Humans, In Vitro Techniques, Inflammasomes, Microscopy, Electron, Transmission, Nanoparticles

Journal Title

Nanomedicine (Lond)

Conference Name

Journal ISSN

1743-5889
1748-6963

Volume Title

10

Publisher

Future Medicine Ltd

Rights

All rights reserved
Sponsorship
Medical Research Council (MR/R005699/1)