Repository logo
 

Cross-species functional diversity within the PIN auxin efflux protein family.

Published version
Peer-reviewed

Type

Article

Change log

Authors

O'Connor, Devin Lee  ORCID logo  https://orcid.org/0000-0003-4071-8626
Ticchiarelli, Fabrizio  ORCID logo  https://orcid.org/0000-0001-5744-2393
Hsia, Mon Mandy 

Abstract

In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated by polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which is conserved in flowering plants. We previously proposed that the AtPIN1 organ initiation and vein patterning functions are split between the SoPIN1 and PIN1 clades in grasses. Here we show that in the grass Brachypodium sopin1 mutants have organ initiation defects similar to Arabidopsis atpin1, while loss of PIN1 function in Brachypodium has little effect on organ initiation but alters stem growth. Heterologous expression of Brachypodium SoPIN1 and PIN1b in Arabidopsis provides further evidence of functional specificity. SoPIN1 but not PIN1b can mediate flower formation in null atpin1 mutants, although both can complement a missense allele. The behavior of SoPIN1 and PIN1b in Arabidopsis illustrates how membrane and tissue-level accumulation, transport activity, and interaction contribute to PIN functional specificity.

Description

Keywords

<i>a. thaliana</i>, Brachypodium, auxin, developmental biology, evo-devo, organ initiation, patterning, plant biology, polarity, stem cells, Arabidopsis, Brachypodium, Genetic Complementation Test, Indoleacetic Acids, Membrane Transport Proteins, Mutation, Plant Growth Regulators, Plant Proteins

Journal Title

Elife

Conference Name

Journal ISSN

2050-084X
2050-084X

Volume Title

6

Publisher

eLife Sciences Publications, Ltd
Sponsorship
Gatsby Charitable Foundation (unknown)
Gatsby Charitable Foundation (GAT3395/CDC)