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Urine proteomics in the diagnosis of stable angina.

Published version
Peer-reviewed

Type

Article

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Authors

Neisius, Ulf 
Koeck, Thomas 
Mischak, Harald 
Rossi, Sabrina H 
Olson, Erin 

Abstract

BACKGROUND: We have previously described a panel of 238 urinary polypeptides specific for established severe coronary artery disease (CAD). Here we studied this polypeptide panel in patients with a wider range of CAD severity. METHODS: We recruited 60 patients who underwent elective coronary angiography for investigation of stable angina. Patients were selected for either having angiographic evidence of CAD or not (NCA) following coronary angiography (n = 30/30; age, 55 ± 6 vs. 56 ± 7 years, P = 0.539) to cover the extremes of the CAD spectrum. A further 66 patients with severe CAD (age, 64 ± 9 years) prior to surgical coronary revascularization were added for correlation studies. The Gensini score was calculated from coronary angiograms as a measure of CAD severity. Urinary proteomic analyses were performed using capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. The urinary polypeptide pattern was classified using a predefined algorithm and resulting in the CAD238 score, which expresses the pattern quantitatively. RESULTS: In the whole cohort of patients with CAD (Gensini score 60 [40; 98]) we found a close correlation between Gensini scores and CAD238 (ρ = 0.465, P < 0.001). After adjustment for age (β = 0.144; P = 0.135) the CAD238 score remained a significant predictor of the Gensini score (β =0.418; P < 0.001). In those with less severe CAD (Gensini score 40 [25; 61]), however, we could not detect a difference in CAD238 compared to patients with NCA (-0.487 ± 0.341 vs. -0.612 ± 0.269, P = 0.119). CONCLUSIONS: In conclusion the urinary polypeptide CAD238 score is associated with CAD burden and has potential as a new cardiovascular biomarker.

Description

Keywords

Coronary artery disease, Stable angina, Urinary proteomics, Angina, Stable, Biomarkers, Coronary Angiography, Coronary Artery Disease, Female, Humans, Male, Middle Aged, Peptides, Predictive Value of Tests, Prognosis, Proteomics, Retrospective Studies, Severity of Illness Index, Urinalysis

Journal Title

BMC Cardiovasc Disord

Conference Name

Journal ISSN

1471-2261
1471-2261

Volume Title

16

Publisher

Springer Science and Business Media LLC