Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts.

The bryophyte Marchantia polymorpha , has attracted significant attention as a powerful experimental system for studying aspects of plant biology including synthetic biology applications. We describe an efficient and simple recursive Type IIS DNA assembly method for the generation of DNA constructs for chloroplast genome manipulation, and an optimized technique for Marchantia chloroplast genome transformation. The utility of the system was demonstrated by the expression of a chloroplast codon-optimized cyan fluorescent protein.

Title

Keywords

Chloroplast, DNA loop assembly, Marchantia, Transformation, Type IIS, Chloroplasts, DNA, Plant, Genetic Engineering, Marchantia, Plants, Genetically Modified, Synthetic Biology, Transformation, Genetic

Is Part Of

Chloroplast Biotechnology: Methods and Protocols

Publisher

Humana

Publisher DOI

https://doi.org/10.1007/978-1-0716-1472-3_21

ISBN

978-1-0716-1472-3

Rights and licensing

Sponsorship

Biotechnology and Biological Sciences Research Council (BB/L014130/1)

Collections

University of Cambridge Research Outputs