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Feasibility of Coacervate-Like Nanostructure for Instant Drug Nanoformulation.

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Azharuddin, Mohammad 
Roberg, Karin 
Biswas, Sujoy Kumar 


Despite the enormous advancements in nanomedicine research, a limited number of nanoformulations are available on the market, and few have been translated to clinics. An easily scalable, sustainable, and cost-effective manufacturing strategy and long-term stability for storage are crucial for successful translation. Here, we report a system and method to instantly formulate NF achieved with a nanoscale polyelectrolyte coacervate-like system, consisting of anionic pseudopeptide poly(l-lysine isophthalamide) derivatives, polyethylenimine, and doxorubicin (Dox) via simple "mix-and-go" addition of precursor solutions in seconds. The coacervate-like nanosystem shows enhanced intracellular delivery of Dox to patient-derived multidrug-resistant (MDR) cells in 3D tumor spheroids. The results demonstrate the feasibility of an instant drug formulation using a coacervate-like nanosystem. We envisage that this technique can be widely utilized in the nanomedicine field to bypass the special requirement of large-scale production and elongated shelf life of nanomaterials.



3D tumor spheroids, coacervate-like nanostructure, instant nanoformulations, nanomedicine, self-assembly, Humans, Feasibility Studies, Doxorubicin, Neoplasms, Nanostructures, Drug Carriers, Nanoparticles, Cell Line, Tumor, Drug Delivery Systems

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ACS Appl Mater Interfaces

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American Chemical Society (ACS)
European Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (706694)