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Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

Stirparo, Giuliano Giuseppe 
Guo, Ge 

Abstract

Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point "mutations" in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.

Description

Keywords

SNP, TP53, cancer-related mutations, genetic integrity, naive pluripotency, pluripotent stem cell, sequencing informatics, single nucleotide polymorphism, Animals, Cell Differentiation, Feeder Cells, Humans, Mice, Mutation, Neoplasms, Pluripotent Stem Cells, Sequence Analysis, RNA

Journal Title

Cell Stem Cell

Conference Name

Journal ISSN

1934-5909
1875-9777

Volume Title

28

Publisher

Elsevier BV
Sponsorship
Medical Research Council (MC_PC_12009)
Medical Research Council (MR/P00072X/1)
Wellcome Trust (203151/Z/16/Z)
Medical Research Council (MC_PC_17230)
We are grateful to James Clarke for cell culture support and to Vicki Murry and Maike Paramor for generating sequencing libraries. This research was funded by the Medical Research Council (MRC) of the United Kingdom. The Wellcome-MRC Cambridge Stem Cell Institute receives core support from Wellcome and MRC. AS is a Medical Research Council Professor.