Repository logo

Heterologous Expression of a Ferritin Homologue Gene PpFer1 from Prunus persica Enhances Plant Tolerance to Iron Toxicity and H2O2 Stress in Arabidopsis thaliana.

Published version

Repository DOI

Change log


Yang, Yong 
Zhang, Jinjin 
Li, Mengyuan 
Ning, Youzheng 
Tao, Yifei 


In plants, ferritin proteins play an important role in iron (Fe) storage which contributes to plant growth and development. However, the biological functions of ferritins in fruit trees are essentially unknown. In this study, three Ferritin genes were isolated from 'Zhentong No. 3' peach, which were named PpFer1-PpFer3. The expression levels of these genes were different in distinct tissues/organs. Notably, PpFer1 was the most abundantly expressed Ferritin family gene in all tested tissues of 'Zhentong No. 3' peach; its expression levels were significantly enhanced throughout the entire peach seedling under Fe toxicity and H2O2 stress, particularly in the leaves. In addition, over-expression of PpFer1 was effective in rescuing the retarded growth of Arabidopsis fer1-2 knockout mutant, embodied in enhanced fresh weight, primary root length, lateral root numbers, total root length, total leaf chlorophyll, stomatal conductance (Gs), net photosynthetic rate (Pn), transpiration rate, and tissue Fe concentration. This study provides insights into understanding the molecular mechanisms of Fe storage and sequestration in perennial fruit trees.


Peer reviewed: True

Acknowledgements: The authors are grateful to grateful to Julia M. Davies, Department of Plant Sciences, University of Cambridge for critical reading and valuable suggestions. The authors are grateful to Irene Murgia, Dipartimento di Biologia, Università degli Studi di Milano, for fer1-2 mutant donation.

Publication status: Published


Fe storage, Fe toxicity, H2O2 stress, ferritin, peach

Journal Title

Plants (Basel)

Conference Name

Journal ISSN


Volume Title



Major Project of Science and Technology of Shandong Province (2022CXGC010605)
China Agriculture Research System of MOF and MARA (CARS-29-17)
China Scholarship Council Fund (202208370080)
Jiangsu Agriculture Science and Technology Innovation Fund (CX(23)3064)
UKRI BBSRC (X008843/1)