Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.


Type
Article
Change log
Authors
Fulara, Aleksandra 
Vandenberghe, Isabel 
Devreese, Bart 
Savvides, Savvas N 
Abstract

The ability of bacteria to infect a host relies in part on the secretion of molecular virulence factors across the cell envelope. Pseudomonas aeruginosa, a ubiquitous environmental bacterium causing opportunistic infections in humans, employs the type II secretion system (T2SS) to transport effector proteins across its cellular envelope as part of a diverse array of virulence strategies. General secretory pathway protein L (GspL) is an essential inner-membrane component of the T2SS apparatus, and is thought to facilitate transduction of the energy from ATP hydrolysis in the cytoplasm to the periplasmic components of the system. However, our incomplete understanding of the assembly principles of the T2SS machinery prevents the mechanistic deconvolution of T2SS-mediated protein secretion. Here we show via two crystal structures that the periplasmic ferredoxin-like domain of GspL (GspLfld) is a dimer stabilized by hydrophobic interactions, and that this interface may allow significant interdomain plasticity. The general dimerization mode of GspLfld is shared with GspL from Vibrio parahaemolyticus suggesting a conserved oligomerization mode across the GspL family. Furthermore, we identified a tetrameric form of the complete periplasmic segment of GspL (GspLperi) which indicates that GspL may be able to adopt multiple oligomeric states as part of its dynamic role in the T2SS apparatus.

Description
Keywords
Amino Acid Sequence, Bacterial Proteins, Models, Molecular, Periplasm, Protein Domains, Protein Multimerization, Protein Structure, Quaternary, Pseudomonas aeruginosa, Type II Secretion Systems
Journal Title
Sci Rep
Conference Name
Journal ISSN
2045-2322
2045-2322
Volume Title
8
Publisher
Springer Science and Business Media LLC
Sponsorship
Wellcome Trust (082961/Z/07/A)
Wellcome Trust (082961/Z/07/Z)
Wellcome Trust (209407/Z/17/Z)