(1)H, (13)C and (15)N resonance assignments of the Cdc42-binding domain of TOCA1.

TOCA1 is a downstream effector protein of the small GTPase, Cdc42. It is a multi-domain protein that includes a membrane binding F-BAR domain, a homology region 1 (HR1) domain, which binds selectively to active Cdc42 and an SH3 domain. TOCA1 is involved in the regulation of actin dynamics in processes such as endocytosis, filopodia formation, neurite elongation, cell motility and invasion. Structural insight into the interaction between TOCA1 and Cdc42 will contribute to our understanding of the role of TOCA1 in actin dynamics. The (1)H, (15)N and (13)C NMR backbone and sidechain resonance assignment of the HR1 domain (12 kDa) presented here provides the foundation for structural studies of the domain and its interactions.

Keywords

Cdc42, Cytoskeleton, Endocytosis, GTPase, HR1, Signalling, TOCA1, Animals, Carrier Proteins, Fatty Acid-Binding Proteins, Nuclear Magnetic Resonance, Biomolecular, Protein Domains, Protein Structure, Secondary, Xenopus, Xenopus Proteins, cdc42 GTP-Binding Protein

Journal Title

Biomolecular NMR Assignments

Journal ISSN

1874-2718
1874-270X

Volume Title

10

Publisher

Springer Nature

Publisher DOI

https://doi.org/10.1007/s12104-016-9677-8

Rights and licensing

Except where otherwised noted, this item's license is described as Attribution 4.0 International

Sponsorship

JRW is supported by a Herchel Smith studentship. The TOCA1 cDNA was a kind gift from Dr. J. L. Gallop.

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Scholarly Works - Biochemistry
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