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Isolation of neural stem and oligodendrocyte progenitor cells from the brain of live rats.

Accepted version
Peer-reviewed

Type

Article

Change log

Authors

McClenahan, Freyja 
Dimitriou, Christina 
Koutsakis, Christos 
Dimitrakopoulos, Dimitrios 
Arampatzis, Asterios 

Abstract

Postnatal brain neural stem and progenitor cells (NSPCs) cluster in anatomically inaccessible stem cell niches, such as the subependymal zone (SEZ). Here, we describe a method for the isolation of NSPCs from live animals, which we term "milking." The intracerebroventricular injection of a release cocktail, containing neuraminidase, integrin-β1-blocking antibody, and fibroblast growth factor 2, induces the controlled flow of NSPCs in the cerebrospinal fluid, where they are collected via liquid biopsies. Isolated cells retain key in vivo self-renewal properties and their cell-type profile reflects the cell composition of their source area, while the function of the niche is sustained even 8 months post-milking. By changing the target area more caudally, we also isolate oligodendrocyte progenitor cells (OPCs) from the corpus callosum. This novel approach for sampling NSPCs and OPCs paves the way for performing longitudinal studies in experimental animals, for more in vivo relevant cell culture assays, and for future clinical neuro-regenerative applications.

Description

Keywords

cerebrospinal fluid, ependymal cell, liquid biopsy, neural stem cells, neuraminidase, neurogenesis, oligodendrocyte progenitor cells, stem cell niche, subependymal zone, ventricular-subventricular zone, Animals, Brain, Cell Culture Techniques, Cell Differentiation, Cerebrospinal Fluid, Corpus Callosum, Humans, Liquid Biopsy, Male, Neural Stem Cells, Oligodendrocyte Precursor Cells, Rats, Rats, Long-Evans, Rats, Sprague-Dawley, Rats, Wistar, Stem Cell Niche

Journal Title

Stem Cell Reports

Conference Name

Journal ISSN

2213-6711
2213-6711

Volume Title

16

Publisher

Elsevier BV

Rights

All rights reserved
Sponsorship
Wellcome Trust (203151/Z/16/Z)
Action Medical Research (GN2291)
Medical Research Council (MC_PC_17230)