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European flow cytometry quality assurance guidelines for the diagnosis of primary immune deficiencies and assessment of immune reconstitution following B cell depletion therapies and transplantation.

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Peer-reviewed

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Authors

Kelleher, Peter 
Greathead, Louise 
Brando, Bruno 
UK NEQAS Leucocyte Immunophenotyping Steering Committee 

Abstract

Over the last 15 years activity of diagnostic flow cytometry services have evolved from monitoring of CD4 T cell subsets in HIV-1 infection to screening for primary and secondary immune deficiencies syndromes and assessment of immune constitution following B cell depleting therapy and transplantation. Changes in laboratory activity in high income countries have been driven by initiation of anti-retroviral therapy (ART) in HIV-1 regardless of CD4 T cell counts, increasing recognition of primary immune deficiency syndromes and the wider application of B cell depleting therapy and transplantation in clinical practice. Laboratories should use their experience in standardization and quality assurance of CD4 T cell counting in HIV-1 infection to provide immune monitoring services to patients with primary and secondary immune deficiencies. Assessment of immune reconstitution post B cell depleting agents and transplantation can also draw on the expertise acquired by flow cytometry laboratories for detection of CD34 stem cell and assessment of MRD in hematological malignancies. This guideline provides recommendations for clinical laboratories on providing flow cytometry services in screening for immune deficiencies and its emerging role immune reconstitution after B cell targeting therapies and transplantation.

Description

Publication status: Published

Keywords

B cell depleting therapy, flow cytometry, hematopoietic cell transplantation, immune reconstitution, primary immune deficiencies, solid organ transplantation

Journal Title

Cytometry B Clin Cytom

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Journal ISSN

1552-4949
1552-4957

Volume Title

Publisher

Wiley