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DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Cannizzaro, Ester 
Bannister, Andrew John 
Alendar, Andrej 

Abstract

DDX3X is a multifunctional RNA helicase with documented roles in different cancer types. Here, we demonstrate that DDX3X plays an oncogenic role in breast cancer cells by modulating the cell cycle. Depletion of DDX3X in MCF7 cells slows cell proliferation by inducing a G1 phase arrest. Notably, DDX3X inhibits expression of Kruppel-like factor 4 (KLF4), a transcription factor and cell cycle repressor. Moreover, DDX3X directly interacts with KLF4 mRNA and regulates its splicing. We show that DDX3X-mediated repression of KLF4 promotes expression of S-phase inducing genes in MCF7 breast cancer cells. These findings provide evidence for a novel function of DDX3X in regulating expression and downstream functions of KLF4, a master negative regulator of the cell cycle.

Description

Keywords

DDX3X, KLF4, RNA helicase, breast cancer, cell cycle

Journal Title

FEBS Letters

Conference Name

Journal ISSN

0014-5793
1873-3468

Volume Title

592

Publisher

Wiley-Blackwell
Sponsorship
Cancer Research Uk (None)
Cancer Research UK (17001)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)