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Rotavirus NSP2: A Master Orchestrator of Early Viral Particle Assembly.

Published version
Peer-reviewed

Repository DOI


Change log

Authors

Nichols, Sarah L 
Borodavka, Alexander  ORCID logo  https://orcid.org/0000-0002-5729-2687
Esstman, Sarah M 

Abstract

Rotaviruses (RVs) are 11-segmented, double-stranded (ds) RNA viruses and important causes of acute gastroenteritis in humans and other animal species. Early RV particle assembly is a multi-step process that includes the assortment, packaging and replication of the 11 genome segments in close connection with capsid morphogenesis. This process occurs inside virally induced, cytosolic, membrane-less organelles called viroplasms. While many viral and cellular proteins play roles during early RV assembly, the octameric nonstructural protein 2 (NSP2) has emerged as a master orchestrator of this key stage of the viral replication cycle. NSP2 is critical for viroplasm biogenesis as well as for the selective RNA-RNA interactions that underpin the assortment of 11 viral genome segments. Moreover, NSP2's associated enzymatic activities might serve to maintain nucleotide pools for use during viral genome replication, a process that is concurrent with early particle assembly. The goal of this review article is to summarize the available data about the structures, functions and interactions of RV NSP2 while also drawing attention to important unanswered questions in the field.

Description

Peer reviewed: True


Acknowledgements: The authors would like to thank members of the Esstman and Borodavka labs for their scientific and editorial suggestions.


Publication status: Published


Funder: Wake Forest Robert and Debra Lee Faculty Fellowship


Funder: MRC-funded Doctoral Training Programme, University of Cambridge

Keywords

genome packaging, genome segment assortment, nonstructural protein 2, particle assembly, replication, rotavirus, viroplasm, Rotavirus, Virus Assembly, Viral Nonstructural Proteins, Humans, Animals, Virus Replication, Genome, Viral, RNA, Viral, Capsid, RNA-Binding Proteins

Journal Title

Viruses

Conference Name

Journal ISSN

1999-4915
1999-4915

Volume Title

16

Publisher

MDPI AG
Sponsorship
NIH (R01-AI116815, T32-AI146059)
Wellcome Trust (103068/Z/13/Z, 213437/Z/18/Z)