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Open questions in human lung organoid research.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Hughes, Tessa 
Dijkstra, Krijn K 
Rawlins, Emma L 

Abstract

Organoids have become a prominent model system in pulmonary research. The ability to establish organoid cultures directly from patient tissue has expanded the repertoire of physiologically relevant preclinical model systems. In addition to their derivation from adult lung stem/progenitor cells, lung organoids can be derived from fetal tissue or induced pluripotent stem cells to fill a critical gap in modelling pulmonary development in vitro. Recent years have seen important progress in the characterisation and refinement of organoid culture systems. Here, we address several open questions in the field, including how closely organoids recapitulate the tissue of origin, how well organoids recapitulate patient cohorts, and how well organoids capture diversity within a patient. We advocate deeper characterisation of models using single cell technologies, generation of more diverse organoid biobanks and further standardisation of culture media.

Description

Keywords

3D cell culture, epithelial cells, in vitro models, lung stem cells, respiratory biology

Journal Title

Front Pharmacol

Conference Name

Journal ISSN

1663-9812
1663-9812

Volume Title

13

Publisher

Frontiers Media SA
Sponsorship
Medical Research Council (MR/P009581/1)
Medical Research Council (MR/R015635/1)
Biotechnology and Biological Sciences Research Council (2114230)