The data comprises of impedance spectra measured using a Sciospec ISX3v2 impedance analyser, of a novel device consisting of aerosol-jet printed silver interdigitated electrodes integrated into a microfluidic channel. Each impedance spectrum (.spec file) consists of some metadata, followed by the measured real (Re / Ohms) and imaginary (Img / Ohms) components measured at each measurement frequency (Freq / Hz). The subfolders contain experimental details. For example, the concentration of the chemical species (in millimolar, mM), and the species. The final subfolder contains information regarding the measurement channel (e.g. '2-4'). Each channel is connected to one pair of electrodes, which lie under 1 channel For temperature-dependent data, a csv file containing times and temperatures is also included. The temperature and impedance spectroscopy measurements were started simultaneously, such that the temperature at which each impedance spectrum was obtained can be determined. csv files are also provided, which have been exported from the code, and contain the impedance spectra and important features (such as the turning point frequency of the capacitance-frequency curves). This enables plotting of the data without running the complete analysis code, if required. The code consists of various Jupyter Notebooks (ipynb) (Python 3), which have been used to analyse the data. Each dataset has a separate associated Jupyter Notebook. The 'source' of the code should be input, and must match the directory of the data on the user's computer. Each function contains a short description, and all functions are called by the 'analysis_all()' function at the end of the script. Comments are present to explain specifics of the code where relevant. Together, the data and code have been used to investigate the effect of anionic species (by comparing sodium chloride (NaCl) and sodium lactate (NaLac), the effect of glucose, and the effect of temperature on the impedance spectra, turning point frequency and Nyquist plots. Nyquist analysis has been carried out to determine the values of the components of the Randles equivalent circuit as a function of temperature for each tested solution.