cam.issuedOnline	2021-10-14
dc.contributor.author	Salas-Leiva, Dayana E
dc.contributor.author	Tromer, Eelco C
dc.contributor.author	Curtis, Bruce A
dc.contributor.author	Jerlström-Hultqvist, Jon
dc.contributor.author	Kolisko, Martin
dc.contributor.author	Yi, Zhenzhen
dc.contributor.author	Salas-Leiva, Joan S
dc.contributor.author	Gallot-Lavallée, Lucie
dc.contributor.author	Williams, Shelby K
dc.contributor.author	Kops, Geert JPL
dc.contributor.author	Archibald, John M
dc.contributor.author	Simpson, Alastair GB
dc.contributor.author	Roger, Andrew J
dc.contributor.orcid	Salas-Leiva, Dayana E [0000-0003-2356-3351]
dc.contributor.orcid	Tromer, Eelco C [0000-0003-3540-7727]
dc.contributor.orcid	Curtis, Bruce A [0000-0001-6729-2173]
dc.contributor.orcid	Jerlström-Hultqvist, Jon [0000-0002-7992-7970]
dc.contributor.orcid	Kolisko, Martin [0000-0003-0600-1867]
dc.contributor.orcid	Yi, Zhenzhen [0000-0002-0693-9989]
dc.contributor.orcid	Salas-Leiva, Joan S [0000-0002-4141-140X]
dc.contributor.orcid	Gallot-Lavallée, Lucie [0000-0001-6763-3388]
dc.contributor.orcid	Williams, Shelby K [0000-0001-7005-5208]
dc.contributor.orcid	Kops, Geert JPL [0000-0003-3555-5295]
dc.contributor.orcid	Archibald, John M [0000-0001-7255-780X]
dc.contributor.orcid	Simpson, Alastair GB [0000-0002-4133-1709]
dc.contributor.orcid	Roger, Andrew J [0000-0003-1370-9820]
dc.date.accessioned	2022-01-05T10:49:51Z
dc.date.available	2022-01-05T10:49:51Z
dc.date.issued	2021-10-14
dc.date.submitted	2021-04-15
dc.date.updated	2022-01-05T10:49:49Z
dc.description	Funder: Canadian Institutes of Health Research (Grant: FRN-142349) Natural Sciences and Engineering Research Council of Canada (Grant: RGPIN 05871-2014)
dc.description.abstract	Cells replicate and segregate their DNA with precision. Previous studies showed that these regulated cell-cycle processes were present in the last eukaryotic common ancestor and that their core molecular parts are conserved across eukaryotes. However, some metamonad parasites have secondarily lost components of the DNA processing and segregation apparatuses. To clarify the evolutionary history of these systems in these unusual eukaryotes, we generated a genome assembly for the free-living metamonad Carpediemonas membranifera and carried out a comparative genomics analysis. Here, we show that parasitic and free-living metamonads harbor an incomplete set of proteins for processing and segregating DNA. Unexpectedly, Carpediemonas species are further streamlined, lacking the origin recognition complex, Cdc6 and most structural kinetochore subunits. Carpediemonas species are thus the first known eukaryotes that appear to lack this suite of conserved complexes, suggesting that they likely rely on yet-to-be-discovered or alternative mechanisms to carry out these fundamental processes.
dc.identifier.doi	10.17863/CAM.79459
dc.identifier.eissn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.other	s41467-021-26077-2
dc.identifier.other	26077
dc.identifier.uri	https://www.repository.cam.ac.uk/handle/1810/332011
dc.language	en
dc.publisher	Springer Science and Business Media LLC
dc.subject	Article
dc.subject	/631/181
dc.subject	/631/326
dc.subject	/45/23
dc.subject	/49/91
dc.subject	article
dc.title	Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.
dc.type	Article
dcterms.dateAccepted	2021-09-14
prism.issueIdentifier	1
prism.publicationName	Nat Commun
prism.volume	12
rioxxterms.licenseref.uri	http://creativecommons.org/licenses/by/4.0/
rioxxterms.version	VoR
rioxxterms.versionofrecord	10.1038/s41467-021-26077-2

Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist.

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