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Hepatitis C Virus in people with experience of injection drug use following their displacement to Southern Ukraine before 2020.

Published version
Peer-reviewed

Repository DOI


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Authors

Yakovleva, Anna 
Kovalenko, Ganna 
Redlinger, Matthew 
Smyrnov, Pavlo 
Tymets, Olga 

Abstract

BACKGROUND: Due to practical challenges associated with genetic sequencing in low-resource environments, the burden of hepatitis C virus (HCV) in forcibly displaced people is understudied. We examined the use of field applicable HCV sequencing methods and phylogenetic analysis to determine HCV transmission dynamics in internally displaced people who inject drugs (IDPWID) in Ukraine. METHODS: In this cross-sectional study, we used modified respondent-driven sampling to recruit IDPWID who were displaced to Odesa, Ukraine, before 2020. We generated partial and near full length genome (NFLG) HCV sequences using Oxford Nanopore Technology (ONT) MinION in a simulated field environment. Maximum likelihood and Bayesian methods were used to establish phylodynamic relationships. RESULTS: Between June and September 2020, we collected epidemiological data and whole blood samples from 164 IDPWID (PNAS Nexus.2023;2(3):pgad008). Rapid testing (Wondfo® One Step HCV; Wondfo® One Step HIV1/2) identified an anti-HCV seroprevalence of 67.7%, and 31.1% of participants tested positive for both anti-HCV and HIV. We generated 57 partial or NFLG HCV sequences and identified eight transmission clusters, of which at least two originated within a year and a half post-displacement. CONCLUSIONS: Locally generated genomic data and phylogenetic analysis in rapidly changing low-resource environments, such as those faced by forcibly displaced people, can help inform effective public health strategies. For example, evidence of HCV transmission clusters originating soon after displacement highlights the importance of implementing urgent preventive interventions in ongoing situations of forced displacement.

Description

Acknowledgements: We acknowledge the assistance of public health workers from the non-governmental organisations “Alliance for Public Health” and “Way Home” in collection of epidemiological and behavioural data and biological samples, and their ongoing efforts of providing preventative measures and infection management to at risk populations in Ukraine. We also express our sincere gratitude towards study participants. We thank Rowena Bull and Chaturaka Rodrigo for their advice with MinION based hepatitis C sequencing protocols.

Keywords

Displacement, Hepatitis C virus, Nanopore sequencing, People who inject drugs, Phylodynamics, Humans, Hepacivirus, Ukraine, Cross-Sectional Studies, Phylogeny, Seroepidemiologic Studies, Bayes Theorem, HIV Infections, Substance Abuse, Intravenous, Hepatitis C, Prevalence

Journal Title

BMC Infect Dis

Conference Name

Journal ISSN

1471-2334
1471-2334

Volume Title

23

Publisher

Springer Science and Business Media LLC
Sponsorship
Wellcome Trust (206298/B/17/Z)
Wellcome Trust (207498/Z/17/Z)