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GC content shapes mRNA storage and decay in human cells.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Courel, Maïté 
Bossevain, Clémentine 
Foretek, Dominika 
Vidal Cruchez, Olivia 

Abstract

mRNA translation and decay appear often intimately linked although the rules of this interplay are poorly understood. In this study, we combined our recent P-body transcriptome with transcriptomes obtained following silencing of broadly acting mRNA decay and repression factors, and with available CLIP and related data. This revealed the central role of GC content in mRNA fate, in terms of P-body localization, mRNA translation and mRNA stability: P-bodies contain mostly AU-rich mRNAs, which have a particular codon usage associated with a low protein yield; AU-rich and GC-rich transcripts tend to follow distinct decay pathways; and the targets of sequence-specific RBPs and miRNAs are also biased in terms of GC content. Altogether, these results suggest an integrated view of post-transcriptional control in human cells where most translation regulation is dedicated to inefficiently translated AU-rich mRNAs, whereas control at the level of 5' decay applies to optimally translated GC-rich mRNAs.

Description

Keywords

GC content, P-bodies, chromosomes, codon usage, gene expression, human, mRNA decay, mRNA storage, post-transcriptional regulation, Base Composition, Gene Expression Regulation, Humans, MicroRNAs, Protein Biosynthesis, RNA Stability, RNA, Messenger, RNA, Messenger, Stored, Transcriptome

Journal Title

Elife

Conference Name

Journal ISSN

2050-084X
2050-084X

Volume Title

8

Publisher

eLife Sciences Publications, Ltd
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/J00779X/1)
Isaac Newton Trust (1507(d))