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Prevalence and polymorphism of a mussel transmissible cancer in Europe.

Accepted version
Peer-reviewed

Type

Article

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Authors

Arbiol, Christine 
Villalba, Antonio 

Abstract

Transmissible cancers are parasitic malignant cell lineages that have acquired the ability to infect new hosts from the same species, or sometimes related species. First described in dogs and Tasmanian devils, transmissible cancers were later discovered in some marine bivalves affected by a leukaemia-like disease. In Mytilus mussels, two lineages of bivalve transmissible neoplasia (BTN) have been described to date (MtrBTN1 and MtrBTN2), both of which emerged in a Mytilus trossulus founder individual. Here, we performed extensive screening of genetic chimerism, a hallmark of transmissible cancer, by genotyping 106 single nucleotide polymorphisms of 5,907 European Mytilus mussels. Genetic analysis allowed us to simultaneously obtain the genotype of hosts - Mytilus edulis, M. galloprovincialis or hybrids - and the genotype of tumours of heavily infected individuals. In addition, a subset of 222 individuals were systematically genotyped and analysed by histology to screen for possible nontransmissible cancers. We detected MtrBTN2 at low prevalence in M. edulis, and also in M. galloprovincialis and hybrids although at a much lower prevalence. No MtrBTN1 or new BTN were found, but eight individuals with nontransmissible neoplasia were observed at a single polluted site on the same sampling date. We observed a diversity of MtrBTN2 genotypes that appeared more introgressed or more ancestral than MtrBTN1 and reference healthy M. trossulus individuals. The observed polymorphism is probably due to somatic null alleles caused by structural variations or point mutations in primer-binding sites leading to enhanced detection of the host alleles. Despite low prevalence, two sublineages divergent by 10% fixed somatic null alleles and one nonsynonymous mtCOI (mitochondrial cytochrome oxidase I) substitution are cospreading in the same geographical area, suggesting a complex diversification of MtrBTN2 since its emergence and host species shift.

Description

Keywords

Mytilus sp. mussels, disseminated neoplasia, genetic chimerism, genetic polymorphism, transmissible cancer, Animals, Dogs, Europe, Mytilus, Mytilus edulis, Neoplasms, Prevalence

Journal Title

Mol Ecol

Conference Name

Journal ISSN

0962-1083
1365-294X

Volume Title

Publisher

Wiley

Rights

All rights reserved
Sponsorship
Montpellier Université d’Excellence (BLUECANCER project), Agence Nationale de la Recherche (TRANSCAN project, ANR-18-CE35-0009), the French DPMA (Direction des pêches maritimes et de l’aquaculture, DPMA-2017-MORBLEU Conventions N°: 16/1212569 & 17/1212952) and Conseil Régional de Normandie, LabEx Team funding).